METABOLIC EFFECTS OF T47D CELLS IN MAMMARY ADIPOSE TISSUE

FRANCISCO DAMIÁN ROSA; IGNACIO AIELLO; MARÍA CECILIA LIRA; IARA CASTELLANOS; ALEJANDRA GRACIELA PALMA; JULIANA LOURDES BERNACCHIA; NATALIA PALADINO; MONICA ALEJANDRA COSTAS; MARÍA FERNANDA RUBIO

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

SAIC

In previous work we have observed that TNF is able to induce lipolysisin adipose tissue (AT) explants from mammary glands ofC57-BL/6J mice and that the breast cancer cell line T47D secreteshigh levels of TNF. The aim of this work was to analyze the metabolicchanges in AT produced by factors secreted for T47D cells.Wild type (wt) or TNF receptor 1 knock out (KO) AT explants werestimulated for 5 days with T47D cell line conditioned media (CM).The number of adipocytes per unit area of paraffin-embedded histologicalsections was measured to assess adipocyte size. The T47DCM induced a decreased in adipocyte size (1.8 ± 0.06 respect to wtbasal, p