Genomic analysis revealed conserved acid tolerance mechanisms from native microorganisms in fermented feed

TERÁN, LUCRECIA C.; MORTERA, PABLO; TUBIO, GISELA; ALARCÓN, SERGIO H.; BLANCATO, VÍCTOR S.; ESPARIZ, MARTIN; ESTEBAN, LUIS; MAGNI, CHRISTIAN

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2022 vol. 132 p. 1152 - 1165

1364-5072

Aims: Fermented feed is an agricultural practice used in many regions of the world to improve the growth performance of farm animals. This study aimed to identify and evaluate the lactic acid bacteria and yeast involved in the production of fermented feed.Methods and Results: We isolated and described two microorganisms from autochthonous microbiota origin present in a regional feed product, Lactobacillus paracasei IBR07 (Lacticaseibacillus paracasei) and Kazachstania unispora IBR014 (Saccharomyces unisporum). Genome sequence analyses were performed to characterize both microorganisms. Potential pathways involved in the acid response, tolerance, and persistence were predicted in both genomes. Though L. paracasei and K. unispora are considered safe for animal feed, we analyzed the presence of virulence factors, antibiotic resistance, and pathogenicity islands. Furthermore, the Galleria mellonella model was used to support the safety of both isolates.Conclusions: We conclude that IBR07 and IBR014 strains are good candidates to be used as starter cultures for feed fermentation. Significance and Impact of the Study: The data presented here will be helpful to explore other biotechnological aspects and constitutes a starting point for further studies to establish the consumption benefit of fermented feed in farm animal production.