Brain changes of serotonergic 5HT2C receptor and oxytocin expression after an acute body sodium depletion

C. PORCARI; MACAGNO AGUSTINA; LAURA VIVAS; ANDREA GODINO

Córdoba

Congreso; XXXIV Reunión Anual SAN 2019; 2019

A temporal dissociation exists between sodium depletion (SD) and the appearance of sodium appetite (SA); and an inhibitory modulation of oxytocinergic and serotonergic (5HT) systems has been postulated. Our recent results also demonstrated a specific temporal pattern of serotonergic and oxytocin receptor mRNA expression, (5HT2C and OTR, respectively), along different brain areas after SD. Our aim was to evaluate 5HT2C and OT proteins expression, during the delay of SA appearance after SD. Wistar rats were SD using furosemide combined with a low sodium diet, and 2h or 24h later were decapitated. Protein samples were extracted of dorsal raphe nucleus (DRN), subfornical organ (SFO), lateral parabrachial (LPBN), paraventricular nucleus (PVN) and supraoptic nucleus (SON), and then were analyzed by western blot. The OT+NP expression increased (p