Beneficial consequences on myocardial mitochondrial network dynamics and function of one-month oral treatment withcannabis oil to spontaneously hypertensive rats (SHR).

PEREYRA E; GODOY COTO J; CAVALLI F; GONZÁLEZ ARBELÁEZ LF; FANTINELLI JC; ARANDA O; COLMAN LERNER E; VELEZ RUEDA O; MOSCA SM; ENNIS IL

Congreso; Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS); 2021

Introduction: Recently, the component of oil cannabis CBDwas foundto regulate mitochondrial DNA epigenetics, biogenesis, and network dynamics.  While abnormal mitochondrial function has beenrepeatedly linked to a wide variety of cardiovascular diseases, components of cannabisoil have proved cardioprotective, attenuating inflammatory and oxidativedamage. However, the effect of cannabis oilon hypertensive cardiac hypertrophy(CH) and specially on mitochondrial function, remains elusive. Objective: To evaluate the consequences of cannabis oil on hypertrophy,mitochondrial function,and mitochondrial networkdynamics in SHR hearts.Methods: Three-month old male SHR were randomized into treated (TR)and control(CR). Cannabis sativa or olive oil were orally administered for 1month, respectively. At the end of treatment, we measured CH, mitochondrial network dynamicsby qPCR, mitochondrial membrane potential by spectrofluorometryand myocardial superoxide dismutase (SOD) activity by spectrometry. Data arepresented as mean±SEM. Welch?s t-test was used for statistical differences(p<0.05). Results: CH, determined by left ventricular weight/tibia lengthratio, was reduced by treatment (mg/mm, CR:33,49±0,2N=6; TR:30,47±0,78N=9,p<0,05). Mitochondria membrane potential was improvedin TR (in mV, CR: -152,9±2,622 N=8, TR:-161,6±2,821N=7, p<0,05).Thetreatment also enhanced mitochondrial biogenesis estimated by mRNA transcripts(%ratio of PG1alpha/GAPDH:CR:100,0 ± 20,33 N=6; TR:156,6 ± 13,80 N=9, p<0,05)and mitochondrial network dynamics(%ratio ofgene/GAPDHexpression:DRP1:CR: 100,0± 10,78 N=5 TR: 330,7 ± 109,3 N=6 p=0.089, MITO1CR:100,0±40,20 N=4; TR:224,1±18,11 N=4, p<0,05, PINK CR:100,0 ± 26,24 N=5,TR:183,3± 20,60 N=8p<0,05) in SHR hearts. Moreover, we observed a significant increasein the activity of the antioxidant SOD by treatment (% of control CR:100,0 ±19,62 N=4, TR:269,4 ± 53,31 N=4p<0,05)Conclusion: We propose that a 1-month treatment with Cannabissativa oil is effective to reduce CH and improve mitochondrial network. Theseeffects may be related to an increased antioxidant capacity.