ISCHEMIC POSTCONDITIONING: DIFFERENCES BETWEEN FEMALE AND MALE

CLAUDIA IRMA CALDIZ; JULIANA CATALINA FANTINELLI; LUISA FERNANDA GONZALE ARBELAEZ; SERGIO SCURI; ALEJANDRO CIOCCI PARDO; SUSANA MARIA MOSCA

Río de Janeiro

Congreso; 38th World Congress of the International Union of Physiological Sciences at the Riocentro Convention Center in Rio de Janeiro; 2017

Ourobjective was to determine the effects of ischemic postconditioning (IPC) on ischemia-reperfusioninjury in male (M) and female (F). Isolated hearts of Wistar rats weresubmitted to the following protocols: 1) Non-ischemic control (NIC): perfusionfor 110 min; 2) Ischemic control (IC): 30-min global ischemia (GI) and 60-minreperfusion (R); 3) IPC: 3 cycles of 30 sec of GI and 30 sec of R at the beginningof R. Infarct size (IS) was measured by TTC technique. Systolic function wasassessed through developed pressure of left ventricle (LVDP) and dP/dtmax;diastolic function by -dP/dtmax and left ventricular end diastolicpressure (LVEDP). At 3 min of R, the expression of phosphorylated forms of Akt,GSK-3β, and PKCε and superoxide production (SP), were determined. In IC, IS waslesser in F ​​thanM (29 ± 2 % and 40 ± 4 %, respectively, p<0.05). At the end of R, LVDP,+dP/dtmax and -dPdtmax were higher in F than M(approximately 55 % and 20 %, respectively, p<0.05); LVEDP was 26±8 mmHg forF and 50±4 mmHg for M (p<0.05). The expression of P-Akt,P-GSK-3β, and P-PKCε in NIC group washigher in F than M and its level increased in both sexes after GI-R. AfterGI-R, SP increased in both sexes but was lesser in F than M in all experimentalgroups. IPC decreased IS and SP in F and M, improved the post-ischemic recoveryof myocardial function and produced additional increase of phosphorylated kinasesexpression only in M.