Neonatal exposure to a glyphosate-based herbicide alters uterine decidualization in rats

INGARAMO PI; VARAYOUD J; MILESI MM; GUERRERO SCHIMPF M; ALARCóN R; MUñOZ-DE-TORO M; LUQUE EH

REPRODUCTIVE TOXICOLOGY (ELMSFORD, N.Y.)

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2017 vol. 73 p. 87 - 95

0890-6238

We investigated whether defective modulation of uterine signaling may cause decidualization failure in rats neonatally exposed to a glyphosate-based herbicide (GBH). Female pups received vehicle or 2 mg/kg of GBH from postnatal day (PND) 1 to PND7. On PND8 and PND21, Wnt5a and â-catenin expression was evaluated in uterine samples. On gestational day (GD) 9, Wnt5a, Wnt7a and â-catenin expression and Dkk1 and sFRP4 mRNA were evaluated on implantation sites. On PND8, GBH-exposed rats showed increased Wnt5a and â-catenin expression in luminal epithelium (LE), whereas on PND21, they showed increased Wnt5a and â-catenin expression in subepithelial stroma but decreased â-catenin expression in glandular epithelium. On GD9, GBH-exposed rats showed decreased Wnt5a and Wnt7a expression in the antimesometrial zone and LE respectively, without changes in â-catenin expression, while Dkk1 and sFRP4 were up- and down-regulated respectively. We concluded that neonatal GBH exposure may lead to embryo losses by disturbing uterine signaling.