Quercetin loaded oil in water microemulsion. Characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1

VERÓNICA VOLPE; S.E. PÉREZ; G. DOLCINI; FERNANDA BUZZOLA; MARCOS GRÜNHUT

Córdoba

Congreso; 6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1); 2021

Universidad Nacional de Córdoba/Universidad Nacional de Rosario

Bovine alphaherpesvirus 1 (BoHV-1) is a virus known to cause several diseases in cattle, including rhinotracheitis, abortion, among others. Quercetin is a flavonoid that presents therapeutic properties such as antioxidant, antibacterial and antiviral. However, the use of quercetin is limited due to its poor water solubility and its limited bioavailability.This work proposes an oil in water microemulsion based on the use of biocompatible materials as a vehicle to load quercetin. The microemulsion was composed of isopropyl myristate (2.50%); tween 80-ethanol (42.0%) and water (55.5%) and was loaded with quercetin at concentrations that ranged from 50-200 mg L-1. Particle size (Z) and polidispersity index (PdI) were measured by dynamic light scattering and antiviral activity studies were performed using MDBK (Madin-Darby bovine kidney) cells and BoHV-1Cooper strain (Titer: 106TCID50mL-1).A significant reduction (p