Lactobacillus plantarum CIDCA 8327: An α-glucan producing-strain isolated from kefir grains

GANGOITI, M.V.; PUERTAS, A.I.; HAMET, M.F.; PERUZZO, P.J.; LLAMAS, M.G.; MEDRANO, M.; PRIETO, A.; DUEÑAS, M.T.; ABRAHAM, A.G.

CARBOHYDRATE POLYMERS

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2017 vol. 170 p. 52 - 59

0144-8617

Lactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producerstrain isolated from kefir with promising properties for the development offunctional foods. The aim of the present study was to characterize thestructure of the EPS synthesized by this strain grown in skim milk orsemidefined medium (SDM). Additionally, genes involved in EPS synthesis weredetected by PCR. L. plantarum produces an EPS with a molecular weight of104 Da in both media. When grown in SDM produce anheteropolysaccharide composed mainly of glucose, glucosamine and rhamnosemeanwhile the EPS produced in milk was composed exclusively of glucoseindicating the influence of the sugar source. FTIR spectra of this EPS showedsignals attributable to an α-glucan. Both by 1H-NMR and methylationanalysis it was possible to determine that this polysaccharide is a branchedα-(1→4)-D-glucan composed of 80% linear α-(1→4)-D-glucopyranosyl units and 19%(1→4)-D-glucopyranosyl units substituted at O-3by single α-D-glucopyranosil residues.