Effects of cadmiun on the expression of beta-catenin, caveolin-1 and HSP27 in ERá(+) and ERá(-) cancer cell lines

SHORTREDE J.; ALVAREZ OLMEDO D.; CUELLO CARRION F. D.; CIOCCA D. R.; FANELLI M. A.

San Luis

Congreso; XXX Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biología de Cuyo

 The metal Cd is a class 1 carcinogen associated with different human tumors. Cd induced disruption of adherents junctions mediated by E-cadherin and induced beta-catenin translocation to the nucleus promoting cell proliferation and survival. In addition, there are interactions of beta-catenin with Hsp27 (HSPB1) and caveolin-1 (cav-1).To evaluate the effects of Cd on the viability, cell survival, apoptosis and localization of  beta-catenin, Hsp27 and cav-1 in ERalpha (+) and ERalpha (-) cancer cell lines.HeLa, MDA-MB-231 and MCF-7 cells were exposed to 1-100  µM CdCl2, for 3 h. We performed MTT, clonogenic assay, immunocytochemistry, TUNEL and Bax/Bcl-2 ratio. Cd treatment reduced the viability of HeLa to 26%, MDA-MB-231 to 84% and MCF-7 to 85%. Colony forming ability was strongly inhibited in HeLa and MCF-7and to a lesser degree in MDA-MB-231. Apoptosis was significantly increased in MCF-7. Cd differentially modulated the distribution of  beta-catenin, cav-1 and Hsp27 in all three cells lines. Protein location is emerging as a key factor to understand tumor cell biology.