NEW FINDINGS ON INDOLE 3-CARBINOL, A PHYTOCHEMICAL DERIVED FROM CRUCIFEROUS. STRONG EVIDENCE OF CARDIOVASCULAR PROTECTIVE EFFECTS

RAMIREZ, D. A.; PRADO, N. J.; MAZZEI, L.; PARRA, M.; CASAROTTO, M.; CALVO, J. P.; CUELLO CARRION, D.; PONCE ZUMINO, A.; DIEZ, E.; MANUCHA W.; CAMARGO A. B

Parma

Conferencia; 3rd International Conference Food Bioactives Health; 2022

The School of Advanced Studies on Food and Nutrition of the University of Parma

I3C (indole-3-carbinol) is a phytochemical found in all types of cruciferous vegetables and demonstrated exceptional anti-cancer effects particularly against hormone-responsive cancers like breast, prostate, and ovarian cancers. Also has been demonstrated potential anti-inflammatory and antioxidant effects. By the way, cardiovascular inflammation and oxidative stress are determining factors in high blood pressure and arrhythmias. However, its implications on the modulation of cardiovascular inflammatory-oxidative markers are unknown. The present study aimed to establish the effects of indole-3-carbinol on the oxidative-inflammatory-proarrhythmic conditions associated with hypertension. Histological, biochemical, molecular, and functional aspects were evaluated in 1) Culture of mouse BV-2 glial cells subjected to oxidative-inflammatory damage by lipopolysaccharides (100 ng/mL) in the presence or absence of 40 μM indole-3-carbinol (n=5); 2) Male spontaneously hypertensive rats (SHR) and Wistar Kyoto rats receiving indole-3-carbinol (2000 ppm/day, orally) during the first 8 weeks of life (n=15); 3) Isolated rat hearts were submitted to 10 min regional ischemia and 10 min reperfusion. Our results showed: 1) lipopolysaccharides induced oxidative stress and increased inflammatory markers; indole-3-carbinol reversed both conditions (interleukin-6, tumor necrosis factor α, the activity of nicotinamide adenine dinucleotide phosphate oxidase, nitric oxide, inducible nitric oxide synthase, heat shock protein, all p