PERSONAL DE APOYO
CUELLO CARRION Fernando Dario
congresos y reuniones científicas
Título:
HSP27 (HSPB1) DOWNREGULATION THROUGH ATR/CHK1 PATHWAY INCREASES CISPLATIN SENSITIVITY IN HUMAN COLON CANCER CELLS
Autor/es:
CAYADO-GUTIÉRREZ N.; REDONDO A. L.; CUELLO CARRION F. D.; LOSSINO A. D.; FANELLI M. A.; VARGAS ROIG L. M.; NADIN S. B.
Lugar:
Connecticut
Reunión:
Simposio; Second Virtual International Symposium on Cellular and Organismal Stress Responses; 2022
Institución organizadora:
Cell Stress Society
Resumen:
HSPB1 has been related to cisplatin (cPt) resistance. We reported that HSPB1interacts with DNA mismatch repair (MMR) colorectal cancer cells. This study aims to investigate HSPB1 and ATR/CHK1 pathway relationship in cPt-exposed HCT116+ch2 (deficient, MMR-) and HCT116+ch3 (proficient, MMR+) cells. HSPB1was downregulated with OGX427 and ATR inhibition by VE-821 (VE). Cells were incubated with 10 μM cPt, 24 h and collected at 0(T0), 3(T3), 9(T9) and 24(T24)h post-treatments. HSP27 nuclear colocalization with CHK1 was demonstrated in cPt-treated MMR-/+ cells. Combined therapy with cPt+OGX427 or cPt+VE reduced cell viability, particularly after cPt+VE in MMR- cells (p
