fficacy and toxicology of Silver Nanoparticles as a component of wound healing dressing in zebrafish embryo and larvae

SOSA AM; IGARTUA DE; GARCIA LR; OCAMPO AG; ALONSO SDV; PRIETO MJ; MARTINEZ CS

Congreso; SAIC 2020; 2020

Silver nanoparticles (AgNPs) have an important role in nanotechnology since theypossess exceptional bactericidal activity. Increasing applications are the AgNP-basedwound dressing, providing a large surface area with high bactericidal efficacy. Weaimed to design a film of hydrogel, composed of autochthonous and low costpolymers. Within the film, oil-in-water emulsions will be used to encapsulate anddelivery AgNPs, vitamins, anesthetic, and anti-inflammatories. This novel device forthe treatment of wounds and burns will be enriched with supplements focused in thereduction of pain and in the acceleration of healing process. As a first step, we willcarry out an analysis of efficacy of the AgNPs in opportunistic bacterias commonlyfound on the skin, and toxicology of the AgNPs in zebrafish embryo and larvae.Results will help to select a concentration to next use in the emulsion. To evaluateeffectiveness of AgNPs, Staphylococcus aureus and Pseudomonas aeruginosa wereincubated with 1.6-60 ppm AgNPs, and minimal inhibitory concentration (MIC) wasdetermined. To evaluate toxicology of AgNPs, Zebrafish embryo and larvae wereincubated with 0.1-100 ppm AgNPs, then studies of LC50, acute toxicity (mortality),teratogenicity (hatching rate and morphology), general developmental abnormalitiesand specific tissue toxicity (brain and heart function) were conducted. The bacterialinhibition indicated a high susceptibility of Staphylococcus aureus and Pseudomonasaeruginosa from 30 ppm. It was observed that AgNPs, in the highest concentrations,caused a delay in the development of embryos, morphological abnormalities in thelarvae, decreased swimming and heart rate. In the larval lethality study, it wasobserved that the LC50 at 1 hs post-incubation was 683 ppm, and at 48 hs post-incubation, it was 108 ppm. The comparison of in vitro and in vivo results allows us tofind a functional and harmless AgNPs dose.