Differential gene expression and hormonal regulation of bone morphogenetic protein inhibitors in bovine oviduct epithelial cells

GARCIA, EV; OLIVA, ME; VALDECANTOS, PA; MICELI, DC ; BARRERA, AD

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Reunión Conjunta de Sociedades de Biociencias

Bone morphogenetic proteins (BMPs) play important roles inmammalian reproduction and female fertility. Previous studies havedemonstrated that BMP ligands and receptors are expressed in ananatomically and temporally regulated fashion in the bovine oviduct.However, the molecular mechanisms that regulate BMP signaling inthe oviductal context are still unknown. The present study aims toexplore the regulatory context of BMP signaling in the bovine oviductby focusing attention on their negative modulators. For this purpose,the mRNA expression of five antagonists of the BMP signaling pathway (FST, BAMBI, NOG, CHRD and SOSTDC1) were evaluated byRT-qPCR in bovine oviduct epithelial cells (BOECs) obtained fromampulla and isthmus at different stages of the estrous cycle. In addition, to evaluate if the expression of these genes respond to hormonal stimulation, cell suspension cultures of BOECs derived fromboth anatomical regions were stimulated with estradiol-17β (0.1, 1and 10 nM) or progesterone (1, 10 and 100 nM) and analyzed byRT-qPCR. The expression of FST, BAMBI, NOG, CHRD and SOSTDC1 was detected in BOECs from ampulla and isthmus during theestrous cycle. Except for NOG, regional and temporal differenceswere observed in the expression patterns of the genes analyzed.Particularly, higher mRNA levels were observed for FST in ampulla (P