NEUTROPHIL CYTOKINE RESPONSES INDUCED BY SHIGA TOXIN (STX)-PRODUCING ESCHERICHIA COLI

SABBIONE FLORENCIA; SHIROMIZU, CAROLINA MAIUMI; KEITELMAN, IRENE ANGÉLICA; VEREERTBRUGGHEN, ALEXIA; ROSSO, DAVID ANTONIO; RAMOS, MARÍA VICTORIA; JANCIC, CAROLINA CRISTINA; GALLETTI, JEREMÍAS; PALERMO, MARINA; ANALÍA, TREVANI SILVINA

Congreso; Reunion de Sociedades de Biociencias 2021; 2021

SAI-SAIC-AAFE-NANOMEDAR

Infections produced by the enteric pathogen shiga toxin (Stx)-producing Escherichia coli (STEC) can cause from diarrhea and hemorrhagic colitis to Hemolytic Uremic Syndrome. STEC are non-invasivebacteria that colonize the intestine where they release the Stx, which then reaches the bloodstream; an event that appears to be facilitated by damages in the intestinal mucosa and promoted byinflammation. As neutrophils (N) are recruited to the intestine upon STEC infections, our aim was to evaluate whether they contribute to the gut inflammatory response by producing cytokines. We previously determined that STEC (E. coli O157:H7 strain), independently of its capacity to produce Stx, stimulates Interleukin-1β (IL-1β) secretion by human N. Here we evaluated the N cytokine responses when challenged with STEC within a range of multiplicities of infection (MOIs: 0.5-10). We found that IL-1β release was higher the lower the MOI was (p