Modulation of gamma delta T lymphocyte functionality by glioblastoma cell line U251

ROSSO, DAVID A; ROSATO, MICAELA; SHIROMIZU, CAROLINA MAIUMI; CORONEL, JUAN VALENTIN; KEITELMAN, IRENE A; SALAMONE, GABRIELA VERÓNICA; JANCIC, CAROLINA C.

CABA

Congreso; Reunión Anual de Sociedades de Biociencias 2020; 2020

Glioblastoma multiforme (GBM) is the most frequent and malignant cause of primary brain tumors in adults; it has poor prognostic, and median survival after diagnostic is less than a year which makes it a top priority for public health. gamma delta T cells are a subtype of T lymphocyte that induce apoptosis on stressed and malignant cells by sensing their increased expression of phosphorylated molecules. Their ability to expand after stimulation and exert cytotoxicity over tumoral cells is being used in immunotherapy approaches against cancer. In this work we aimed to evaluate the modulation of gamma delta T cell functionality by human GBM cell line U251 and how it could impact in their antitumoral response. For that purpose, gamma delta T cells were purified from human peripheral blood mononuclear cells, by using an anti-TCR gamma delta MicroBead isolation kit. After purification, gamma delta T cells were co-cultured with U251 cell line or it?s conditioned medium for 24 hours in the absence or presence of phosphoantigen (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP, 1 μM). After incubation, we analyzed the activation state of gamma delta T cells by measuring the expression of CD69 and perforin by flow cytometry, and TNF-alpha and IFN-gamma production by ELISA. Our results indicated that HMBPP-stimulated gamma delta T cells exhibit an increase in CD69 expression (p