Modulation of gammadelta T cell funciton by neutrophils

SABBIONE, FLORENCIA; GABELLONI, MARÍA LAURA; ERNST, GLENDA; GORI, MARÍA SOLEDAD; SALAMONE, GABRIELA; ANALÍA, TREVANI; GEFFNER, JORGE; JANCIC, CAROLINA

Los Cocos

Congreso; 61 Reunión Anual de la Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de Inmunología

gd T cells constitute a functionally specialized subset of T lymphocytes which play an important role in linking the innate and adaptive immunity. Moreover, gd T cells modulate the function of neutrophils; growing evidence supports a critical role of gd T cells in the recruitment and activation of neutrophils at the sites of infection through the release of a range of cytokines and chemokines. In this study we analyzed whether neutrophils were able to modulate the phenotype and function of human blood gd T cells. We found that the up-regulation of CD25 (p<0.05, n=22) and CD69 (p<0.05, n=7) expression, the production of IFN-g (p<0.05, n=6), and the proliferation of gd T cells (p<0.05, n=7) induced by HDMAPP were inhibited by neutrophils. It is well known that neutrophils produce immunoregulatory molecules such as prostaglandin E2, arginase I, and reactive oxygen species (ROS). We found that inhibitors of prostaglandin E2 and arginase I did not exert any effect, by contrast, catalase prevented the suppression of gd T cell activation induced by neutrophils (p<0.05, n=5) suggesting a role for hydrogen peroxide. Our results reveal a bidirectional cross-talk between gd T cells and neutrophils. While gd T cells promote the recruitment and the activation of neutrophils to fight invading pathogens, neutrophils in turn might suppress the activation of gd T cells to contribute to the resolution of inflammation.