Graft recovery mediated by diallyl disulfide during cold storage in a non-heart beating rat liver donation model

BALABAN CECILIA L.; RODRIGUEZ JOAQUIN V.; GUIBERT EDGARDO E.

Natal

Congreso; VII Congreso Latino Americano de Órganos Artificiales y Biomateriales (COLAOB); 2012

Sociedad Latino Americana de Biomateriales, Ingeniaría de Tejidos y Órganos Artificiales (SLABO)

Liver transplantation is currently the preferred treatment option for end-stage liver disease, reaching that status because of the continuous improvement in the organ transplantation field. Non-heart beating donation (NHBD) was employed in the early years of organ donation, before brain death criteria were established. These organs are subjected to variable periods of warm ischemia that may intensify cold ischemia/reperfusion injuries. However, due to shortage of brain death donors, the use of NHB grafts is being reconsidered. The present work deals with the setting of a rat model of liver donation after cardiac death and the assessment of a preservation solution additive, diallyl disulfide (DADS, garlic derived,) thought to have cytoprotectant properties mediated by H2S. Cardiopulmonary arrest was induced in a sedated animal by an I.V. injection of KCl/Heparin and warm ischemia period extended up to 45 minutes. Livers were surgically removed and subsequently stored in Custodiol® HTK Solution (Histidine-tryptophan-ketoglutarate) at 0-4 °C. Experimental groups were the following: I- Heart Beating Donated (HBD) preserved 24 hs in HTK; II- NHBD preserved 24 hs in HTK; III- NHBD preserved 24 hs in HTK + 5 µM DADS. Intrahepatic Resistance (IR60: mmHg.min.gliv/mL) and Perfusion Flow (PF60: mL/min.gliv) values showed liver microvasculature damage in NHBD grafts (group II*: IR 2.88±0.55 PF 2.41±0.71) when compare to livers from HBD (group I: IR 2.17±0.11 PF 3.39±0.17) (*p