CONICET | Buscador de Institutos y Recursos Humanos

To identify the transcriptional mechanisms underpinning Fusarium head blight (FHB) resistance in durum wheat, a RNA-seq comparative transcriptome analyses was conducted on infected spikes of the resistant line Langdon(Dic-3A)10 and Langdon susceptible variety, 72 hours post inoculation. In a previous study, 14 differential expressed genes (DEGs) were mapped in the quantitative trait locus Qfhs.ndsu-3AS region, associated with type II resistance to FHB. Here, we focused on the global response of durum wheat to Fusarium graminearum (Fg) infection, identifying the molecular pathways in which DEGs are involved and lncRNAs that interplay in the transcriptional and post-transcriptional regulation of gene expression. Using the reference genome of cv. Svevo, 921 genes and 22 lncRNA were differentially expressed. A set of 452 DEGs were up-regulated in Langdon(Dic-3A)10. According to MapMan software, the resistant genotype showed the overexpression of genes involved in receptors kinases signaling, phytohormone pathway, calcium signaling, secondary metabolites biosynthesis, sensitivity to toxins, fungal cell-wall degrading and transcription factors. The differentially expressed lncRNAs showed different genome locations: 12 cis intergenic (6 up-regulated in Langdon(Dic-3A)10), 4 trans intergenic (one up-regulated in Langdon(Dic-3A)10), 4 intronic (two up-regulated in Langdon(Dic-3A)10) and 2 putative miRNAs precursors. For 14 lncRNAs, the possible target genes were predicted and showed potential regulative effects on wax and lignin synthesis and ankyrines in the resistant line. In a context where resistance sources against Fg are scarce in durum germplasm, the transcriptomic characterization of a valuable genotype contributes to understand the genetic bases and the regulatory network involved in resistance conferred by Qfhs.ndsu-3AS.

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