Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of low-level laser therapy in experimental myopathy

SERVETTO, NATALIA; CREMONEZZI, DAVID; SIMES, JUAN; MOYA, MÓNICA; SORIANO, FERNANDO; PALMA, JOSE ATILIO; CAMPANA, VILMA

LASERS IN SURGERY AND MEDICINE

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2010 vol. 42 p. 577 - 583

0196-8092

The objective of the present work was to study the effect of helium–neon (He–Ne) and gallium–arsenide (Ga–As) laser upon inflammatory biomarkers associated with oxidative stress: fibrinogen, nitric oxide (NO), L-citrulline, and superoxide dismutase (SOD). These were evaluated through histological assessment, in rats with experimental myopathy. Materials and Methods: The groups studied were: (A) control, (B) injured, (C) injured and treated with He–Ne laser, (D) injured and treated with Ga–As laser, (E) irradiated with He–Ne; and (F) irradiated with Ga–As laser. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left posterior limb muscle at the same point on 5 consecutive days, in groups B, C, and D. Lowlevel laser therapy (LLLT) was applied with 9.5 J/cm2 daily for 7 consecutive days with each laser. The determination of the biomarkers was made by spectrophotometry. The muscles (5/8, single blinded) were stained with Gomori Trichrome and examined by optic microscopy. The quantitative variables were statistically analyzed by the Fisher’s test and categorical data by the Axionvision 4.8 program. Pearson’s chi-squared test was applied, setting significant difference at P