Regulation of FGF2-induced cell proliferation by inhibitory GPCR in normal pituitary cell

SOSA L DEL V.; PICECH, F; ZLOCOWSKI N; DE PAUL, A L; PETITI J P; TORRES, A I

Buenos Aires

Congreso; ICE2021Virtual-19th International congress of Endocrinology-4th Latin American Congress of Endocrinology (CONLAEN)-13th Congress of the Argentine Federation of Endocrinology Societies (FASEN); 2021

Introduction: The growth factors effects may be regulated by the inhibitory G protein-coupled receptors (GPCR-Gαi) activation, thus modifying the metabolic activity of pituitary gland. Objectives: Considering that intracellular communications are essential; the aim was to evaluate whether GPCR-Gαi regulates the basic fibroblast growth factor (FGF2) proliferative activity in normal pituitary cell populations. Methods: Anterior pituitary primary cell cultures from Wistar female rats were treated with FGF2 (10ng/mL) or somatostatin analogue (OCT, 100nM) alone or co-incubated with or without an inhibitor of GPCR-Gαi, pertussis toxin (PTX, 500nM) or MEK inhibitor (U0126, 100µM). Results: The BrdU uptake shows that the basal lactotroph and somatotoph mitosis cells was 6.9% and 3.34%, respectively. The FGF2 increased and OCT decreased the lactotroph and somatotroph BrdU positive cells respect to control group (p