INVESTIGADORES
SOSA Liliana Del Valle
artículos
Título:
17b-estradiol stimulates the translocation of endogenous estrogen receptor alpha at the plasma membrane of normal anterior pituitary cells
Autor/es:
GUTIERREZ, S.; SOSA LDEL, V.; PETITI, J. P.; MUKDSI, J. H.; MASCANFRONI, I.; PELLIZAS,C.; DE PAUL, A. L.; CAMBIASO, M J; TORRES, A. I.
Revista:
MOLECULAR AND CELLULAR ENDOCRINOLOGY.
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Año: 2012 vol. 355 p. 169 - 179
ISSN:
0303-7207
Resumen:
In the present work we aimed at identifying ERalpha in the plasma membrane of normal anterior pituitary cells and investigated if 17b-estradiol was able to induce their subcellular redistribution. Our results show that about 8% of anterior pituitary cells expressed ERα in the plasma membrane, with the geometrical mean fluorescence intensity being increased after steroid hormone treatment. 17b-Estradiol and the selective ERα agonist PPT induced an increase of ERα expression in the plasma membrane and activated the PKCalpha/ERK 1/2 pathway in a time-course not compatible with genomic actions, thus supporting the notion of membrane-initiated effects. These findings suggest that 17b-estradiol stimulates the translocation of endogenous ERa to the plasma membrane, consequently modulating this ER pool and leading to cellular biological effects in normal anterior pituitary gland.
