Effect of the Lack of Post-Pubertal, Ovary-Dependent Programming on Metabolic-Endocrineand Liver Functions

VILLAGARCÍA H; CASTRO MC; MASSA ML; SPINEDI E; FRANCINI F

Orlando

Congreso; ENDO2017 The Endocrine Society´s 99th annual meeting; 2017

Background & Aim: Post-pubertal bilateral ovariectomy (OVX)-induced Metabolic Syndrome (MS)phenotype in rats is characterized by increased body weight (BW)/fat and endocrine-metabolicdysfunctions. Our aim was to evaluate the effect of the lack of pubertal programming, by the endogenouspeak of ovary-derived estradiol (E2), on endocrine-metabolic and liver functions when adulthood (90 daysold) was reached. Materials and Methods: Sprague-Dawley rats were either OVXon day 23/25 of life(pre-pubertal OVX; ppOVX) or sham (ppSHX) operated (dorsal approach) under mild anesthesia.Recovered rats were allocated 3 per cage, with free access to rat chow and water up to 69 days of age;then divided in groups either receiving drinking water (C) or fructose rich diet (FRD; 10% F in drinkingwater and rat chow provided ad libitum during 3 weeks). Built groups were: ppSHX-C, ppOVX-C, ppSHX-Fand ppOVX-F. Subsets of these groups were sacrificed in non-fasting (basal) condition or, after overnightfasting, submitted to an ip glucose tolerance test (GTT; 2 g/kg BW). BWs, food-intake and dranksolution were recorded day after day. Circulating levels of glucose (GLU), triglyceride (TG) and estradiol(E2) were quantified. Glycemias were monitored throughout (0-120 min) the GTT, and individual areaunder the curve (AUC) of circulating GLU levels was calculated. Finally, liver gene expression levels oflipogenesis-related proteins (SREBP-1c, FAS and GPAT) and estrogen receptor-A (ER-A) werequantified. Results: Non-fasting ppOVXrats showed (p