Fatty liver and insulin resistance: time course appearance of the changes induced by a fructose rich diet

MASSA ML; BLAIOTTA C; CASTRO MC; GAGLIARDINO JJ; FRANCINI F

Berli;n

Congreso; 48th EASD annual meeting; 2012

EASD

Background and aims: we have previously shown that administration of fructose to normal rats during 3 weeks induced insulin resistance, impaired glucose tolerance, fatty liver and hepatic carbohydrate metabolism abnormalities. The aim of this study was to evaluate the time-course of development of these alterations triggered by a fructose rich diet (FRD). Materials and methods: for this purpose we fed normal male Wistar rats for 7, 14 and 21 days with a) a standard commercial diet (C) and b) a C diet plus 10% fructose in the drinking water (DRF). At each time we measured glycemia (G), triglyceridemia (TG) and insulinemia (I). HOMA-IR, HOMA-beta and Index for hepatic sensitivity to insulin (IHIS) ratios were calculated. In liver we measured a) protein carbonyl groups and reduced glutathione (GSH) as oxidative stress markers (OSM), b) liver glycogen and triglyceride content, c) glucokinase (GK), glucose-6-phosphatase (G-6-Pase) and glucose-6-phosphate dehydrogenase (G-6-P DH) activities and d) SREBP-1c, fatty acid synthase (FAS), glycerol-3-phosphate acyltransferase (GPAT), G-6-Pase and G-6-P DH gene expression. Results: (*p