INVESTIGADORES
CURATTI Leonardo
artículos
Título:
Sucrose is involved in the diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp.
Autor/es:
CURATTI, LEONARDO; FLORES, ENRIQUE; SALERNO GRACIELA
Revista:
FEBS LETTERS
Referencias:
Año: 2002 vol. 13 p. 175 - 178
ISSN:
0014-5793
Resumen:
Sucrose synthase (SuS)
expression was studied in the filamentous, nitrogen-fixing cyanobacterium
Anabaena sp. PCC 7119. SuS activity, SusA polypeptide, and susA mRNA levels
were lower in cells cultured diazotrophically than in the presence of combined
nitrogen. An insertional susA mutant presented a dramatic increase in sucrose
levels, whereas the disaccharide was not detectable in a susA overexpressing
strain, indicating that SusA is involved in the cleavage of sucrose in vivo.
Diazotrophic growth was impaired in the susA overexpressing strain, suggesting
a role for sucrose in diazotrophic metabolism and the involvement of SusA in
the control of carbon flux in the N(2)-fixing filament.