Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: Identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli

CURATTI, L.; FOLCO, E.; DESPLATS, P.; ABRATTI, G.; LIMONES V.; HERRERA-ESTRELLA, L.; SALERNO, G.

JOURNAL OF BACTERIOLOGY

Año: 1998 vol. 180 p. 6776 - 6779

0021-9193

The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties.