Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren?s syndrome patients

ENRI BORDA, SABRINA GANZINELLI; SILVIA REINA

International Journal of Dentistry and Oral Health

Sci Forschen

Lugar: New York; Año: 2016 vol. 2 p. 123 - 129

2471-657X

Aims: The purpose of this report is to describe saliva IgA antibody against M3muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren?s syndrome (pSS). Inaddition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in wholesaliva of pSS patients.Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patientswith pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-merpeptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined byELISA.Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negativepatients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased inoptical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was usedas coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit theaction when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together inthe microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ropositive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects.Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than thoseIgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significantdecrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects.