INVESTIGADORES
CAVELLO Ivana Alejandra
congresos y reuniones científicas
Título:
Biodegradation of a keratin waste and the concomitant production of detergent stable serine proteases from Paecilomyces lilacinus
Autor/es:
CAVELLO, IA; CAVALITTO,S.F; HOURS, RA
Lugar:
Trivandrum
Reunión:
Congreso; International Conference on New Horizons in Biotechnology and 8 th Annual Convention of The Biotech Research Society, India.; 2011
Institución organizadora:
National Institute for Interdiciplinary Science and Technology, CSIR, Trivandrum. The Biotech Research Society, India.
Resumen:
Paecilomyces lilacinus strain LPS # 876, isolated from
alkaline soils from Buenos Aires Province (Argentine), efficiently degraded
feather keratin during submerged cultivations, producing extracellular
proteases. The present study describes the characterization of crude protease
and its compatibility in commercial detergents. The optimum pH and temperature
for proteolytic activity were 10.0 and 60°C, respectively. Protease activity
was enhanced in the presence of 1 mM Ca2+ and Mg2+, but was strongly inhibited
by PMSF and by Hg2+ suggesting the presence of thiol-dependent serine
proteases. The crude protease showed extreme stability toward non-ionic (5%
Tween 20; 5% Tween 85 and 5% Triton X-100) and anionic (1% SDS) surfactants,
and relative stability toward various oxidizing agents. In addition, it showed
excellent stability and compatibility with different solid (7 mg/ml) and liquid
(1% v/v) commercial detergents for 1 h at temperatures from 30 to 50°C. The
enzyme preparation retained more than 95% of its initial activity after
pre-incubation at 40°C with solid detergents (Ariel and Drive followed by
Skip and Ala). It retained 97% of its original activity after pre-incubation
at 40°C with a liquid detergent (Ace). The protective effect of polyols (propylene
glycol, PEG 4000 and glycerol) on the heat inactivation was also examined and
the best results were obtained with glycerol from 50 to 60°C.
Considering its promising properties, P. lilacinus enzymatic
preparation may be considered a potential candidate for future use in
biotechnological processes, particularly as detergent additive and in the
processing of poultry wastes, particularly keratinous wastes.