BETA-CYCLODENTRIN COATED MAGNETIC NANOPARTICLES: NEW INSIGHTS FOR THE LOCALIZED TREATMENT OF DIVERSE PATHOLOGIES

AGOTEGARAY MARIELA; GARCÍA ELVA; CAMPELO ADRIÁN; MASSHEIMER VIRGINIA; LASSALLE VERÓNICA

Mar del Plata

Congreso; LXI Reunión Científica anual de la Sociedad Argentina de investigación Clínica.; 2016

Sociedad Argentina de Investigación Clínica

Introduction Magnetic nanoparticles (MNPs) provide new in-sights for targeted drug delivery to aspecific site in the organism by an external magnetic field. Coating of MNPs improvesbiocompat-ibility and provides a platform to attach drugs for diverse purposes. Objective Synthesis, characterization and evaluation of endo-thelial cytotoxicity of nano-systemscomposed of magnetite (MG), oleic acid(OA) and betacyclodextrin (BCD) as nano-devices for drug targeting. Design OA stabilized MG MNPs were synthesized by co-precipitation. A dispersion of MAG-OA (0.5 mg/mL) in hexane was mixed with aqueous solution of BCD in different MNPs:BCD ratios (1:1; 1:2; 1:3, named MG-OA-BCD1, MG-OA-BCD2, MG-OA-BCD3) for 24h at room temperature. The organic phase was separated; the content of the aqueous phase was extracted with Nd magnet, washed and dried. Samples were studied by FTIR, DLS to determine hydrodynamic diameter (Dh) and surface charge(z); TEM and HR-TEM. Primary cultures of Rat aortic endothelial cells (EC)were exposed 48h to final concentrations of 1, 10 and 100 µg/mL of MG-OA-BCD1 and MG-OA-BCD2. Cell viability was evaluated by MTT assay and by the capacity to produce NO (DAN assay). Results FTIR demonstrated the incorporation of BCD on MG-OA. Aqueous monodisperse MG-OA-BCD1, MG-OA-BCD2, MG-OA-BCD3 presented Dh of 589.3±42.71nm, 370.3±6.00nm, 555.4±8.50nm and z of 2.27±6.14mV, 10.3±4.85mV, 14.4±5.12mV respectively. HR-TEM micrographs showed almost-spherical shaped MNPs. Synthesis of MG-OA-BCD3 was not reproducible. Cell viability was not affected at doses of 10µg/mL for MG-OA-BCD1 and 1 µg/mL for MG-OA-BCD2(p<0.001). Basal NO produc-tion was not altered at any of the concentrations for both MNPs. Conclusion The synthesis of nano MAG-OA-BCD was success-ful. Physicochemical properties depend on BCD concentration. EC cytotoxicity of MNPs was dose dependent with no adverse effects at concentrations below 10µg/ml. The novel nanosystems may possibly be suitable for biomedical applications.