SUPERANTIGEN INDUCE APOPTOSIS IN HUMAN NEOPLASTIC T CELLS

DUARTE ALEJANDRA; PASTORINI MERCEDES; SCARFO MARINA; ROMAN RISO ALEJANDRO; ALEMÁN MERCEDES

Mar del Plata

Congreso; Sociedad Argentina de Investigción Clínica; 2018

Superantigens (Sags) are bacterial and virus protein that share the ability to activate large number of T-cells. Sags bind to major histocompatibility complex (MHC) class II molecules as unprocessed proteins and interact with T cells expressing particular T-cell receptor (TCR) Vβ chains. We have previously shown that bacterial and mouse mammary tumor virus (MMTV)-encoded Sags induced apoptosis of different murine-cognate lymphoma T cells both in vitro and in vivo. Moreover, bacterial T Sags increased the survival of lymphoma-bearing mice. Furthermore, we have recently described that Sags were also able to induce apoptosis of the Jurkat-established human cell line from an acute T-carrier leukemia of the Vβ8 region in TCR. Thus, we now aimed to evaluate the effect of Sags on xenografts of Jurkat Cells in mice. For this propose we subcutaneously inoculated Jurkat cells (5x106) on Scid/Nod mice and then we treated them intraperitoneally with specific Sag SEE (50µg). Afterward, we analyzed the tumor growth and V8+ cells in blood weekly for 6 weeks when final weight and histology were evaluated. Results: While SEE treatment did not affect immunological parameters or general fitness of mice, the tumor growth as well as lymphocyte number in blood, were significantly reduced at week 5 and 4 respectively (p