IDENTIFICATION OF MULTIPLE DIFFERENTIALLY EX¬PRESSED MESSENGER RNAs IN HUMAN GESTATIONAL TROPHOBLASTIC DISEASES (GTDs)

ABADIE, P.; DURAND, S.: ANGELETTI, S. AND GENTI-RUIMONDI. S.

Córdoba

Congreso; XIII Annual Scientific Meeting, Cordoba Biology Society. CORDOBA - ARGENTINA; 2001

SAIB

Gestational trophoblastic diseases comprise a group ofinterrelated neoplasms, including partial hydatidiform mole, complete hydatidiform mole, placental site trophoblastic tumor and choriocarcinoma. During the past few years the molecular and biochemical character­ization of distinct gene expression repertoires and or the specific products has emphasized the functional importance of differentially expressed genes in establishing normal or tumoral phenotypes. A search for genes involved in the pathogenesis and prognosis of gestational tumors applying Differential Display techniques allowed us to isolate several differentially expressed cDNA fragments. Pre­viously, we reported that three of these fragments corresponded to mitochondrial transcripts with diminished expression in both be­nign and malignant trophoblastic tumors (Placenta. 22:220. 2001 ) In addition, the differential expression of decorin, hnRNP A1 and ribosomal L 27 was communicated. In the present report, we ex­tended the molecular characterization to other three cDNA fragments. Two of them were upregulated in JEG-3 choriocarcinoma cell line and the other one was upregulated in normal early pla­centa. Cloning experiments and sequence data showed that two of them corresponded to known mRNA: light ferritin and ribosomal L26. One of the sequences corresponded to transcript of yet-uni­dentified gene. A search for a full length sequence is currently per­formed. Taken together, these data demonstrate that significant changes in the transcription of nuclear and mitochondrial genomes is associated with the phenotypic alteration present in GTDs.