IN VIVO EFECTS OF FRACTIONS OBTAINED FROM Larrea divaricata ON MACROPHAGES

MARTINO R; DAVICINO RC; CASALI YA, ; CASALI YA; MATTAR MA; MICALIZZI B

Huerta Grande-

Congreso; Sociedad de Biología de Córdoba; 2009

Sociedad de Biología de Córdoba

Larrea divaricata Cav is a plant known in Argentina for its use on folk medicine to treat different diseases. The aim of this work was to evaluate in vivo effect of three fractions (F1, F2 and F3) obtained from an aqueous extract on murine macrophages. Mouse were injected intraperitoneally twice in a 48 hs period with 0,5; 5 and 15 mg/kg of each fraction. Peritoneal cells were obtained 96 hs after first injection and were harvested during 2 hs at 37ºC. The following determinations were carried out: 1) cellular viability by MTT test; 2) phagocytosis by NBT reductions and 3) apoptosis by: a) Giemsa staining and b) ethidium bromide/ acridine orange staining. The results showed that a significant increased (p<0.05) of the cellular viability occur with F2 (15 mg/kg) compared with control. A no significant difference was observed with the other fractions. A significant increase (p<0.05) of the phagocytosis with F2 (15 mg/kg) and F3 (5 and 15mg/kg) was observed. A significant increase of the apoptosis (p< 0.05) was observed when Giemsa was used, with F1 (15mg/kg), F2 (0.5mg/kg) and F3 (15mg/kg). In conclusion, F2 at 15 mg/kg showed a cellular viability and phagocytosis increased, and an anti-apoptotic effect too, as well as F3 increase la NBT reduction at high concentrations, possibly for its NDGA content. No effects were observed with F1 in vivo on macrophages, only an anti-apoptotic activity.is a plant known in Argentina for its use on folk medicine to treat different diseases. The aim of this work was to evaluate in vivo effect of three fractions (F1, F2 and F3) obtained from an aqueous extract on murine macrophages. Mouse were injected intraperitoneally twice in a 48 hs period with 0,5; 5 and 15 mg/kg of each fraction. Peritoneal cells were obtained 96 hs after first injection and were harvested during 2 hs at 37ºC. The following determinations were carried out: 1) cellular viability by MTT test; 2) phagocytosis by NBT reductions and 3) apoptosis by: a) Giemsa staining and b) ethidium bromide/ acridine orange staining. The results showed that a significant increased (p<0.05) of the cellular viability occur with F2 (15 mg/kg) compared with control. A no significant difference was observed with the other fractions. A significant increase (p<0.05) of the phagocytosis with F2 (15 mg/kg) and F3 (5 and 15mg/kg) was observed. A significant increase of the apoptosis (p< 0.05) was observed when Giemsa was used, with F1 (15mg/kg), F2 (0.5mg/kg) and F3 (15mg/kg). In conclusion, F2 at 15 mg/kg showed a cellular viability and phagocytosis increased, and an anti-apoptotic effect too, as well as F3 increase la NBT reduction at high concentrations, possibly for its NDGA content. No effects were observed with F1 in vivo on macrophages, only an anti-apoptotic activity.in vivo effect of three fractions (F1, F2 and F3) obtained from an aqueous extract on murine macrophages. Mouse were injected intraperitoneally twice in a 48 hs period with 0,5; 5 and 15 mg/kg of each fraction. Peritoneal cells were obtained 96 hs after first injection and were harvested during 2 hs at 37ºC. The following determinations were carried out: 1) cellular viability by MTT test; 2) phagocytosis by NBT reductions and 3) apoptosis by: a) Giemsa staining and b) ethidium bromide/ acridine orange staining. The results showed that a significant increased (p<0.05) of the cellular viability occur with F2 (15 mg/kg) compared with control. A no significant difference was observed with the other fractions. A significant increase (p<0.05) of the phagocytosis with F2 (15 mg/kg) and F3 (5 and 15mg/kg) was observed. A significant increase of the apoptosis (p< 0.05) was observed when Giemsa was used, with F1 (15mg/kg), F2 (0.5mg/kg) and F3 (15mg/kg). In conclusion, F2 at 15 mg/kg showed a cellular viability and phagocytosis increased, and an anti-apoptotic effect too, as well as F3 increase la NBT reduction at high concentrations, possibly for its NDGA content. No effects were observed with F1 in vivo on macrophages, only an anti-apoptotic activity.in vivo on macrophages, only an anti-apoptotic activity.