Cross-reaction between proteins of Larrea divaricata Cav. and proteins of slime-negative or slime-positive Staphyloccocus epidermidis.

DÁVILA S, ; RAGUSA JAV; , MATTAR MA,; DAVICINO RC,; MARTINO R; CASALI YA,; MICALIZZI B

Ciudad de la Punta, San Luis

Congreso; XXVII Reunión Anual Científica de la Sociedad de Biología de Cuyo; 2009

Sociedad de Biología de Cuyo

Larrea divaricata (jarilla) is an abundant plant of the Northwest of Argentine with a documented folk use related to antimicrobial and antitumoral activities. Staphyloccocus epidermidis is important as the main pathogen in foreign body infections, and it has emerged as a leading cause of nosocomial infections. S. epidermidis is considered as a pathogen for its ability to colonize specially when medical devices implanted are used as well as its ability to form biofilms. The aim of this study was to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (JPCE) of jarilla. We evaluated the cross reaction between JPCE and cellular proteins (CP) obtained by sonication of S. epidermidis strains. Slime-negative (ATCC 12228) and slime-positive (clinical) strains were used. Mice sera of anti- JPCE were used. Protein profiles of JPCE and CP were analyzed by SDS-PAGE. Levels of IgG anti-JPCE against CP were determined by ELISA. JPCE showed 18 bands (from 20 to 176 kDa). We find a percentage of similarity between the protein profiles of JPCE and CP (35% approximately). CP showed cross reaction with the anti-JPCE serum. The mean titers of anti-JPCE against CP of ATCC 12228 or clinical S. epidermidis strains were >1/100 and >1/200 respectively. Although these results it is necessary to continue searching to identify and purify the antigens responsible of this cross reaction with jarilla.(jarilla) is an abundant plant of the Northwest of Argentine with a documented folk use related to antimicrobial and antitumoral activities. Staphyloccocus epidermidis is important as the main pathogen in foreign body infections, and it has emerged as a leading cause of nosocomial infections. S. epidermidis is considered as a pathogen for its ability to colonize specially when medical devices implanted are used as well as its ability to form biofilms. The aim of this study was to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (JPCE) of jarilla. We evaluated the cross reaction between JPCE and cellular proteins (CP) obtained by sonication of S. epidermidis strains. Slime-negative (ATCC 12228) and slime-positive (clinical) strains were used. Mice sera of anti- JPCE were used. Protein profiles of JPCE and CP were analyzed by SDS-PAGE. Levels of IgG anti-JPCE against CP were determined by ELISA. JPCE showed 18 bands (from 20 to 176 kDa). We find a percentage of similarity between the protein profiles of JPCE and CP (35% approximately). CP showed cross reaction with the anti-JPCE serum. The mean titers of anti-JPCE against CP of ATCC 12228 or clinical S. epidermidis strains were >1/100 and >1/200 respectively. Although these results it is necessary to continue searching to identify and purify the antigens responsible of this cross reaction with jarilla.Staphyloccocus epidermidis is important as the main pathogen in foreign body infections, and it has emerged as a leading cause of nosocomial infections. S. epidermidis is considered as a pathogen for its ability to colonize specially when medical devices implanted are used as well as its ability to form biofilms. The aim of this study was to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (JPCE) of jarilla. We evaluated the cross reaction between JPCE and cellular proteins (CP) obtained by sonication of S. epidermidis strains. Slime-negative (ATCC 12228) and slime-positive (clinical) strains were used. Mice sera of anti- JPCE were used. Protein profiles of JPCE and CP were analyzed by SDS-PAGE. Levels of IgG anti-JPCE against CP were determined by ELISA. JPCE showed 18 bands (from 20 to 176 kDa). We find a percentage of similarity between the protein profiles of JPCE and CP (35% approximately). CP showed cross reaction with the anti-JPCE serum. The mean titers of anti-JPCE against CP of ATCC 12228 or clinical S. epidermidis strains were >1/100 and >1/200 respectively. Although these results it is necessary to continue searching to identify and purify the antigens responsible of this cross reaction with jarilla.S. epidermidis is considered as a pathogen for its ability to colonize specially when medical devices implanted are used as well as its ability to form biofilms. The aim of this study was to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (JPCE) of jarilla. We evaluated the cross reaction between JPCE and cellular proteins (CP) obtained by sonication of S. epidermidis strains. Slime-negative (ATCC 12228) and slime-positive (clinical) strains were used. Mice sera of anti- JPCE were used. Protein profiles of JPCE and CP were analyzed by SDS-PAGE. Levels of IgG anti-JPCE against CP were determined by ELISA. JPCE showed 18 bands (from 20 to 176 kDa). We find a percentage of similarity between the protein profiles of JPCE and CP (35% approximately). CP showed cross reaction with the anti-JPCE serum. The mean titers of anti-JPCE against CP of ATCC 12228 or clinical S. epidermidis strains were >1/100 and >1/200 respectively. Although these results it is necessary to continue searching to identify and purify the antigens responsible of this cross reaction with jarilla.is considered as a pathogen for its ability to colonize specially when medical devices implanted are used as well as its ability to form biofilms. The aim of this study was to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (JPCE) of jarilla. We evaluated the cross reaction between JPCE and cellular proteins (CP) obtained by sonication of S. epidermidis strains. Slime-negative (ATCC 12228) and slime-positive (clinical) strains were used. Mice sera of anti- JPCE were used. Protein profiles of JPCE and CP were analyzed by SDS-PAGE. Levels of IgG anti-JPCE against CP were determined by ELISA. JPCE showed 18 bands (from 20 to 176 kDa). We find a percentage of similarity between the protein profiles of JPCE and CP (35% approximately). CP showed cross reaction with the anti-JPCE serum. The mean titers of anti-JPCE against CP of ATCC 12228 or clinical S. epidermidis strains were >1/100 and >1/200 respectively. Although these results it is necessary to continue searching to identify and purify the antigens responsible of this cross reaction with jarilla.S. epidermidis strains. Slime-negative (ATCC 12228) and slime-positive (clinical) strains were used. Mice sera of anti- JPCE were used. Protein profiles of JPCE and CP were analyzed by SDS-PAGE. Levels of IgG anti-JPCE against CP were determined by ELISA. JPCE showed 18 bands (from 20 to 176 kDa). We find a percentage of similarity between the protein profiles of JPCE and CP (35% approximately). CP showed cross reaction with the anti-JPCE serum. The mean titers of anti-JPCE against CP of ATCC 12228 or clinical S. epidermidis strains were >1/100 and >1/200 respectively. Although these results it is necessary to continue searching to identify and purify the antigens responsible of this cross reaction with jarilla.S. epidermidis strains were >1/100 and >1/200 respectively. Although these results it is necessary to continue searching to identify and purify the antigens responsible of this cross reaction with jarilla.