CROSS-REACTION INDUCED BY Larrea divaricata Cav. CRUDE EXTRACT AGAINST Pseudomonas aeruginosa CELLULAR ANTIGENIC PREPARATIONS

RAGUSA JAV, ; MATTAR MA, .; DAVICINO RC,; MARTINO R,; CASALI YA,; MICALIZZI B

Ciudad de la Punta, San Luis

Congreso; XXVII Reunión Anual Científica de la Sociedad de Biología de Cuyo.; 2009

Sociedad de Biología de Cuyo

Larrea divaricata Cav. (jarilla) is a plant with a documented folk use related to antimicrobial and antitumoral activities. However, there are few articles about cross-reaction between jarilla and use related to antimicrobial and antitumoral activities. However, there are few articles about cross-reaction between jarilla and Cav. (jarilla) is a plant with a documented folk use related to antimicrobial and antitumoral activities. However, there are few articles about cross-reaction between jarilla and Pseudomonas aeruginosa cellular antigens. The aim of this study was evaluate the humoral response of L. divaricata crude extract (JPCE) against different cellular antigens of P. aeruginosa ATCC 27853, and to analyze antigenic similarities by SDS-PAGE. Mice of Rockland strain weighing 18-20 g were subcutaneously (s.c.) injected with JPCE. IgG response was evaluated by ELISA. The proteins from sonicated cell, cytoplasm and membrane were used as bacterial antigens. When anti-JPCE sera were tested against the bacterial antigens significative differences (p<0.01) was observed between IgG titer from sonicated cell and membrane proteins respect cytoplasm ones. The higher proteic similarity was observed between L. divaricata and P. aeruginosa membrane (35%). These results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. 27853, and to analyze antigenic similarities by SDS-PAGE. Mice of Rockland strain weighing 18-20 g were subcutaneously (s.c.) injected with JPCE. IgG response was evaluated by ELISA. The proteins from sonicated cell, cytoplasm and membrane were used as bacterial antigens. When anti-JPCE sera were tested against the bacterial antigens significative differences (p<0.01) was observed between IgG titer from sonicated cell and membrane proteins respect cytoplasm ones. The higher proteic similarity was observed between L. divaricata and P. aeruginosa membrane (35%). These results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. (JPCE) against different cellular antigens of P. aeruginosa ATCC 27853, and to analyze antigenic similarities by SDS-PAGE. Mice of Rockland strain weighing 18-20 g were subcutaneously (s.c.) injected with JPCE. IgG response was evaluated by ELISA. The proteins from sonicated cell, cytoplasm and membrane were used as bacterial antigens. When anti-JPCE sera were tested against the bacterial antigens significative differences (p<0.01) was observed between IgG titer from sonicated cell and membrane proteins respect cytoplasm ones. The higher proteic similarity was observed between L. divaricata and P. aeruginosa membrane (35%). These results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. 27853, and to analyze antigenic similarities by SDS-PAGE. Mice of Rockland strain weighing 18-20 g were subcutaneously (s.c.) injected with JPCE. IgG response was evaluated by ELISA. The proteins from sonicated cell, cytoplasm and membrane were used as bacterial antigens. When anti-JPCE sera were tested against the bacterial antigens significative differences (p<0.01) was observed between IgG titer from sonicated cell and membrane proteins respect cytoplasm ones. The higher proteic similarity was observed between L. divaricata and P. aeruginosa membrane (35%). These results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. was evaluate the humoral response of L. divaricata crude extract (JPCE) against different cellular antigens of P. aeruginosa ATCC 27853, and to analyze antigenic similarities by SDS-PAGE. Mice of Rockland strain weighing 18-20 g were subcutaneously (s.c.) injected with JPCE. IgG response was evaluated by ELISA. The proteins from sonicated cell, cytoplasm and membrane were used as bacterial antigens. When anti-JPCE sera were tested against the bacterial antigens significative differences (p<0.01) was observed between IgG titer from sonicated cell and membrane proteins respect cytoplasm ones. The higher proteic similarity was observed between L. divaricata and P. aeruginosa membrane (35%). These results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. 27853, and to analyze antigenic similarities by SDS-PAGE. Mice of Rockland strain weighing 18-20 g were subcutaneously (s.c.) injected with JPCE. IgG response was evaluated by ELISA. The proteins from sonicated cell, cytoplasm and membrane were used as bacterial antigens. When anti-JPCE sera were tested against the bacterial antigens significative differences (p<0.01) was observed between IgG titer from sonicated cell and membrane proteins respect cytoplasm ones. The higher proteic similarity was observed between L. divaricata and P. aeruginosa membrane (35%). These results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. (JPCE) against different cellular antigens of P. aeruginosa ATCC 27853, and to analyze antigenic similarities by SDS-PAGE. Mice of Rockland strain weighing 18-20 g were subcutaneously (s.c.) injected with JPCE. IgG response was evaluated by ELISA. The proteins from sonicated cell, cytoplasm and membrane were used as bacterial antigens. When anti-JPCE sera were tested against the bacterial antigens significative differences (p<0.01) was observed between IgG titer from sonicated cell and membrane proteins respect cytoplasm ones. The higher proteic similarity was observed between L. divaricata and P. aeruginosa membrane (35%). These results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. 27853, and to analyze antigenic similarities by SDS-PAGE. Mice of Rockland strain weighing 18-20 g were subcutaneously (s.c.) injected with JPCE. IgG response was evaluated by ELISA. The proteins from sonicated cell, cytoplasm and membrane were used as bacterial antigens. When anti-JPCE sera were tested against the bacterial antigens significative differences (p<0.01) was observed between IgG titer from sonicated cell and membrane proteins respect cytoplasm ones. The higher proteic similarity was observed between L. divaricata and P. aeruginosa membrane (35%). These results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. cellular antigens. The aim of this study was evaluate the humoral response of L. divaricata crude extract (JPCE) against different cellular antigens of P. aeruginosa ATCC 27853, and to analyze antigenic similarities by SDS-PAGE. Mice of Rockland strain weighing 18-20 g were subcutaneously (s.c.) injected with JPCE. IgG response was evaluated by ELISA. The proteins from sonicated cell, cytoplasm and membrane were used as bacterial antigens. When anti-JPCE sera were tested against the bacterial antigens significative differences (p<0.01) was observed between IgG titer from sonicated cell and membrane proteins respect cytoplasm ones. The higher proteic similarity was observed between L. divaricata and P. aeruginosa membrane (35%). These results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. divaricata Cav. extract and different cellular antigens from P. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. aeruginosa. This study suggests that proteins of L. divaricata could potentially be used in the manufacturing of human vaccines. potentially be used in the manufacturing of human vaccines. results demonstrate the presence of common antigens between L. divaricata Cav. extract and different cellular antigens from P. aeruginosa