ANTIGEN SIMILARITY BETWEEN Larrea divaricata Cav.PROTEINS AND Pseudomonas aeruginosa CELLULAR AND EXTRACELLULAR PROTEINS

SASSO, C; RAGUSA JAV; MATTAR MA; DAVICINO R; MARTINO R,; CASALI; MICALIZZI B.

Ciudad de la Punta, San Luis

Congreso; XXVII Reunión Anual Científica de la Sociedad de Biología de Cuyo; 2009

Sociedad de Biología de Cuyo

Larrea divaricata Cav. commonly known as jarilla, has been used to treat of a number of conditions. Nevertheless, no information is available so far as regards immunological properties. Pseudomonas aeruginosa is a Gram negative bacilli isolated from different environments mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa is a Gram negative bacilli isolated from different environments mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and treat of a number of conditions. Nevertheless, no information is available so far as regards immunological properties. Pseudomonas aeruginosa is a Gram negative bacilli isolated from different environments mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa is a Gram negative bacilli isolated from different environments mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and Cav. commonly known as jarilla, has been used to treat of a number of conditions. Nevertheless, no information is available so far as regards immunological properties. Pseudomonas aeruginosa is a Gram negative bacilli isolated from different environments mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa is a Gram negative bacilli isolated from different environments mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and mainly from hospitals, being considered a nosocomial pathogen. The aim of this study was to compare proteins from crude extract of jarilla (JPCE) and cellular and extracellular bacterial proteins. JPCE was obtained from leaves in PBS 7,4, 24 hs at 4ºC and concentrated by ultrafiltration. The microorganism used was P. aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and aeruginosa ATCC 27853; the cellular proteins were obtained by sonication and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein profiles of the three antigens showed a similarity coefficient greater than 40%. The antibodies anti JPCE showed a cross reactivity with cellular and extracellular antigens (p< 0,05) in relation to the negative control. The results obtained showed an immunological similarity between crude extract of L. divaricata Cav and and the exoproducts were obtained from the supernatant, dialyzed, and lyophilized. To determine cross reactivity an anti sera JPCE against the bacterial antigens was tested The levels of IgG were obtained by using an ELISA test. Extract and bacterial protein samples were electrophoresed through a 10% separating polyacrylamide gel. The protein pro