EFECTOS INMUNOMODULADORES IN VITRO DE UN EXTRACTO ACUOSO DE LARREA DIVARICATA CAV. EN CÉLULAS DENDRÍTICAS Y EPITELIALES HUMANAS

ROBERTO DAVICINO; INéS TISCORNIA; VIVIANA SáNCHEZ; CLAUDIA ANESINI; MARIELA BOLLATI-FOGOLíN

Montevideo

Congreso; IV LATIN AMERICAN SEMINAR ON CELL CULTURE TECHNOLOGY (IV SLATCC); 2010

IP de Montevideo

Larrea divaricata (Zygophyllaceae) is a South American  plant  widely distributed in Argentina. It is used in folk medicine for treatment of arthritis, inflammation, gastric disturbance, venereal diseases, tuberculosis and in healing sores and wounds. It possesses a well documented immunomodulatory, anti-inflammatory and antitumoral action in cells derived from rats. Dendritic cells are essential components of innate immunity that regulate the development of adaptative immune response and also collaborate in the modulation of T cells responses.  By other way, HT 29-NFêB-hr GFP cells are used to evaluate anti-inflammatory and antitumoral actions of drugs. The aim of this study was to study the immunomodulatory, anti-inflammatory and antitumoral actions of an aqueous extract of the plant (AE) on dendritic cells induced from human monocytes of peripheral blood and on human HT 29-NFêB-hr GFP.  On dendritic cells were studied: phagocytosis, cytokines production ( IL8, IL6 and IL10),  and HLA-DR, CD86 and HLA-DR/CD86 expression (by flow cytometry) and in HT29 cells, cytokines (IL-8) ( by flow citometry) , cell viability ( by MTT assay), apoptosis ( by flow cytometry with annexin) and  NFêB translocation by GFP fluorescein expression. In dendritic cells: AE did not modify phagocytosis but increased the expression of HLA-DR/C86 and IL8, IL6 and IL10 production. In HT cells: AE decreased the translocation of NFêB and IL8 production induced by TNFa. Also decreased cell viability and induced cells to apoptosis. It can be concluded that, AE exerted an immunodulatory effects on dendritic cells, promoting cell activation by the production of anti and pro-inflammatory cytokines. Moreover, exerted antitumoral activity on HT tumor cells as well as demonstrated anti-inflammatory action. These results confirmed the immunomodulatory action of Larrea divaricata   on human cells.