Fatty acid profile and salivary flow in fasted rats

ESCANDRIOLO NACKAUZI J; GALLARÁ R; REPOSSI G; ACTIS A

Santiago de Chile

Congreso; IV Reunión Región Latinoamericana de la International Association for Dental Research.; 2011

International Association for Dental Research

Saliva is a secretion that plays a key role in the integrity of the hard and soft tissues of the oral cavity. Unstimulated saliva -mainly of submandibular origin and secreted between meals- cleans, lubricates and provides substances that help to repair and maintain that integrity. OBJECTIVE: To analyze the effect of fasting (F) on salivary flow (SF) and fatty acid profile (FA) in submandibular (Sm) saliva of adult rats. METHODS: adult male Wistar rats were used. They were divided into a 16-hour fasting (F) and a non-fasting (nF) groups. After anesthesia, secretory ducts from both submandibular glands were exposed and cannulated with fine glass tubes to obtain submandibular saliva. To induce the salivary secretion, a mixture of isoproterenol and pilocarpine (5 mg/kg body weight) was injected intraperitoneally. Then, the saliva was collected for 20 minutes. The samples were frozen at -20º C until processing. Salivary lipids were extracted by Folch?s method and the FAs were methylated with sodium methoxide. The methylesters obtained were determined by gas chromatography. Their values were expressed as a percentage of area. The Student´s t test was employed to compare the proportion of each FA between the two groups (p