MODULATION OF SOME ENDOCANNABINOID SYSTEM (ECS) COMPONENTS AND CELL PROLIFERATION BY EXOGENOUS FATTY ACIDS IN THREE HUMAN CANCER CELL LINES

REPOSSI G; EYNARD AR; DAS UN

Huerta Grande, Córdoba

Jornada; XVII Jornadas Científicas de la Sociedad de Biología de Córdoba; 2009

Sociedad de Biología de Córdoba

Introduction:  dietary  unsatturated  fatty  acids   (UFA)  may modulate cancer  development  and ECS.  Aim:  to analyse  the effects of  UFAs 20:5n3 (EPA), 20:4n6 (AA), 18:1 (OA) and satturated   16:0 (PA) on MCF7,  T24 and T98G cancer  lines proliferation and ECS receptors. Methodology:  the   cells   lines  MCF7   (breast   cancer),  T24   (urinary bladder   carcinoma)   and  T98G  (neuroblastoma)  were   cultured   and supplemented with each FA essayed. Cell proliferation was evaluated by MTT method,  CB  receptors  by ELISA  and FA  cell  profiles   in membranes   by   GLC.   Data   obtained   were   statistically   analysed. Results: MTT: AA and EPA suppresses cell proliferation in T98G and MCF7   respectively;   whereas   PA  and   OA  stimulate   cell   growth. ELISA: slight increases on the immunodetection of CB2 receptors in MCF7 and T98 were observed. GLC: we observed a fairy reasonable correspondence between exogenous UFAs and the enrichment of FA in membranes composition. Discussion: this results show a differential modulation by FA supplementation on cell proliferation, CB receptors expression and membrane FA profiles in differents cell cancer lines. The effects of FA have been associated with ERK kinase activation (Soto-Guzman  et   al,   2008)   and   eicosanoids   and  endocannabinoids metabolism,  metabolites  FA derived,  capables  of  modify growth  in cancer cells (Pasqualini et al, 2008)