INVESTIGADORES
REPOSSI MARQUEZ Pablo Gaston
artículos
Título:
The parasite Trypanosoma cruzi alter the trofoblast (regeneration of Sincytiotrophoblast) in human term placenta in vitro
Autor/es:
DÍAZ LUJÁN C; TRIQUELL MF; REPOSSI G; QUIROGA P; PETS E; BOLATTI E; FRETES R
Revista:
UNLaR Ciencia
Editorial:
UNIVERSIDAD NACIONAL DE LA RIOJA
Referencias:
Lugar: La Rioja, Argentina; Año: 2007 vol. 1 p. 13 - 18
ISSN:
1515-5005
Resumen:
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Because placental chorionic villi may act as a
barrier to the causal agent of Chagas disease, the effect of Trypanosoma cruzi
on the trophoblast turnover were analyzed in chorionic villi denuded of their
syncytiotrophoblast (STB). Three chorionic villi explants (30 mg) from
placentas at term were denuded of STB with trypsin. Explants were co-cultured
with trypomastigotes of Tulahuen and Lucky (isolated of congenital Chagas
disease) strains for 72h. Explants were processed for structural studies and
immunohistochemistry for Nitric Oxide Synthase III (eNOS), Alkaline Phosphatase
(PLAP) and E-cadherin. Proliferation of cytotrophoblast (CTB) was measured
(Axiovision 3.0.6), and the concentration of hCG (mlU/ml), PLAP activity (μmol of p-nitrophenol/mg protein/min).
In the denuded explants the Lucky strain showed
less percentage of the CTB (0.28%) and a diminished level of hCG (309±26.87)
than Tulahuen (p<0.05). Lucky
culture media showed higher level of PLAP activity, 3.3±0.49, than Tulahuen
ones, , 0.27±0.09.
CTB proliferation was affected by T. Cruzi, and
this effect was more pronounced by the congenital strain. These data, together
with the decreased hCG levels and dysfunction of STB, suggest that the
trophoblast turnover is altered by the parasite and that this process may
account for the congenital transmission and the low birth weight associated to
congenital Chagas disease.
