Genetic variability study in 19 bovine breeds of five candidate genes related with marbling (DGAT1, TG, GH, LP and SCD).

ROGBERG-MUÑOZ, A; RIPOLI, MV; VILLEGAS-CASTAGNASSO, EE; KIENAST, ME; FURNUS, CC; PERAL-GARCÍA, P; GIOVAMBATTISTA, G

Porto Seguro, Brazil.

Conferencia; 30th International Conference on Animal Genetics; 2006

International Society for Animal Genetics

The use of Simple Nucleotide Polymorphisms (SNPs) in genetic characterization and Marker Assisted Selection (MAS) has increased in the last years. Marbling is one of the most important traits that influence the meat international market price; this fact makes it suitable for MAS programs. In the present study, the polymorphisms of five SNPs of candidate genes related with marbling were analyzed: Diacilglicerol aciltransferase 1 (DGAT1), tyroglobulin (TG), leptin (LP), growth hormone (GH) and stearoyl-coa desaturase (SCD). Samples from more than 400 animals of 19 Bos taurus (European and Creole) and Bos indicus breeds were collected. The DNA was analyzed by PCR-RFLP or PCR-SSCP. The results identify one or two alleles present in each studied population. Statistically significant variation was observed in the allele frequencies and expected heterocigocity between them. The distribution of these frequencies could be explained by the breeds’ phylogenetic origin. DGAT1 distribution shows European and Cebuine breeds in each extreme and Creole, Retinta, Jersey and Wagyu breeds in an intermediate position. The TG genes presented a distribution with Cebuine and beef European breeds in one extreme, Jersey and Wagyu in the other, and Creole and Holstein in the middle. The extremes of GH locus distribution were occupied by Cebuine breeds, Holstein and Normande in one side and Wagyu on the other, appearing the rest of European breeds in the middle and the Creole distributed all over. The monomorphism of SCD was present in most breeds. Finally, LP locus distribution did not allowed the classification of breeds in separate groups.