Ultrastructural study of echinococcus granulosus cysts treated in vivo with an abz-cyclodextrin solution.

ELISSONDO, C; CEBALLOS, L; DOPCHIZ, M; ANDRESIUK, V; ALVAREZ, L; SANCHEZ BRUNI, S; LANUSSE, C; DENEGRI, G

CÓRDOBA. Argentina

Congreso; XXXVIII Reunion Anual de la Asociación Argentina de Farmacología Experimantal; 2006

Albendazole (ABZ), currently used for chemotherapeutic treatment of cystic hydatid disease, is usually formulated as a suspension. The aim of this work was to study the ultrastructural alterations produced in vivo by an ABZ-cyclodextrin solution on E. granulosus cysts using scanning and transmission electron microscopes (SEM and TEM). BalbC mice were intraperitoneally infected with protoscoleces (1500/animal). A year after infection, mice were divided in two groups (n= 10): 1) control, untreated; 2) orally treated with a ABZ-cyclodextrin solution (500 ìM) for 50 days. After treatment, animals were euthanised and the cysts were weighed and subjected to ultrastructure study. No significant difference between control and treated group was found related to the weight of cyst masses (P > 0.05). All cysts removed from control mice appeared turgid and no alteration in ultrastructure was detected. Cysts from the treated group revealed at TEM alterations in the germinal layer with the presence of numerous vacuoles. At SEM, only cellular debris of the germinal layer was observed. MODALIDAD  POSTER