INVESTIGADORES
CEBALLOS Laura
artículos
Título:
Comparative assessment of albendazole and triclabendazole
Autor/es:
ALVAREZ, L; MORENO, L.; MORENO, G; CEBALLOS, L; SHAW, L; FAIRWEATHER, I
Revista:
VETERINARY PARASITOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2009 p. 211 - 216
ISSN:
0304-4017
Resumen:
The benzimidazole compounds albendazole (ABZ) and triclabendazole (TCBZ) are both
effective against Fasciola hepatica, although ABZ is only effective against adult flukes.
Additionally, ABZ is a broad-spectrum nematodicidal compound with well-known ovicidal
activity. However, no data on the ovicidal effect of TCBZ against F. hepatica eggs are
available. The work reported here evaluated the comparative ovicidal effect of ABZ, TCBZ
and their sulphoxide metabolites on F. hepatica eggs recovered from bile of sheep
artificially infected with either a TCBZ-susceptible (Cullompton) or a TCBZ-resistant (Sligo)
isolate of F. hepatica. Additionally, the effects of different non-flukicidal methylcarbamate
benzimidazole compounds on the hatching of F. hepatica eggs were evaluated. Eggs
(500 eggs/mL, n = 4) were incubated for 12 h either with TCBZ, TCBZ sulphoxide (TCBZ.SO),
ABZ (5, 10 and 20 nmol/mL) or without drug (untreated control) (Experiment 1).
Additionally, the effect of TCBZ and TCBZ.SO (5 nmol/mL) on egg hatchability was
examined after a long (15 days) drug exposure (Experiment 2). Furthermore, the ovicidal
effect of ABZ and ABZ.SO at different concentrations (5, 1, 0.5, 0.1and 0.05 nmol/mL)
(Experiment 3), and the effect of fenbendazole (FBZ), oxfendazole (OFZ), mebendazole
(MBZ), flubendazole (FLBZ) (5 nmol/mL) and reduced-FLBZ (R-FLBZ) (2 mg/mL) on fluke
eggs, were evaluated after a 12-h exposure (Experiment 4). Egg hatch was assessed by
direct microscopic observation after incubation at 25 8C for 15 days. TCBZ and TCBZ.SO did
not affect egg hatch after a 12-h incubation. A similar result was obtained after a much
longer drug exposure (15 days) (Experiment 1 and 2). However, a significant (P < 0.05)
inhibition of egg hatch was observed in ABZ- and ABZ.SO-incubated eggs (Experiments 1
and 3). Additionally, the non-flukicidal compounds (Experiment 4) affected egg
hatchability, particularly FLBZ and R-FLBZ. In conclusion, ABZ and ABZ.SO had a clear
inhibitory effect on egg development of F. hepatica. However, the most extensively used
flukicidal compound, TCBZ, and its main sulphoxide metabolite, did not affect egg hatch,
even in TCBZ-susceptible flukes.