INVESTIGADORES
CEBALLOS Laura
artículos
Título:
Flubendazole and ivermectin in vitro combination therapy produces a marked effect on Echinococcus granulosus protoscoleces and metacestodes
Autor/es:
ELISSONDO, M; CEBALLOS, L; ALVAREZ, L; SANCHEZ BRUNI, S; LANUSSE, C; DENEGRI, G
Revista:
PARASITOLOGY RESEARCH
Editorial:
SPRINGER
Referencias:
Año: 2009 p. 835 - 842
ISSN:
0932-0113
Resumen:
Abstract The aim of the present work was to evaluate
the in vitro efficacy of the flubendazole (FLBZ) and
ivermectin (IVM) combination against Echinococcus
granulosus protoscoleces and metacestodes. Protoscoleces
and groups of ten peritoneal cysts obtained from BALB/c
mice were incubated with the two drugs, either separately
or in combination, at the following final concentrations:
10 ìg/mL FLBZ, 1 ìg/mL FLBZ, 1 ìg/mL IVM, 10 ìg/
mL FLBZ + 1 ìg/mL IVM, and 1 ìg/mL FLBZ + 1 ìg/
mL IVM. The maximum protoscolicidal effect was found
with the combination 10 ìg/mL FLBZ + 1 ìg/mL IMV.
After 1 day of incubation, the presence of numerous blebs
in the tegument of protoscoleces was observed. Ultrastructural
studies revealed that the primary site of damage
was the tegument of the parasite. The effect of the two
drugs on hydatid cysts obtained from mice was more
rapidly detected in cysts treated with the combination of
FLBZ + IVM than when drugs were used separately.
Ultrastructural studies revealed that the germinal layer of
treated cysts lost the multicellular structure feature and
underwent considerable degenerative changes after in
vitro treatment. The outcomes obtained demonstrated the
favorable effect of the combination of FLBZ and IVM
against E. granulosus.The aim of the present work was to evaluate
the in vitro efficacy of the flubendazole (FLBZ) and
ivermectin (IVM) combination against Echinococcus
granulosus protoscoleces and metacestodes. Protoscoleces
and groups of ten peritoneal cysts obtained from BALB/c
mice were incubated with the two drugs, either separately
or in combination, at the following final concentrations:
10 ìg/mL FLBZ, 1 ìg/mL FLBZ, 1 ìg/mL IVM, 10 ìg/
mL FLBZ + 1 ìg/mL IVM, and 1 ìg/mL FLBZ + 1 ìg/
mL IVM. The maximum protoscolicidal effect was found
with the combination 10 ìg/mL FLBZ + 1 ìg/mL IMV.
After 1 day of incubation, the presence of numerous blebs
in the tegument of protoscoleces was observed. Ultrastructural
studies revealed that the primary site of damage
was the tegument of the parasite. The effect of the two
drugs on hydatid cysts obtained from mice was more
rapidly detected in cysts treated with the combination of
FLBZ + IVM than when drugs were used separately.
Ultrastructural studies revealed that the germinal layer of
treated cysts lost the multicellular structure feature and
underwent considerable degenerative changes after in
vitro treatment. The outcomes obtained demonstrated the
favorable effect of the combination of FLBZ and IVM
against E. granulosus.Echinococcus
granulosus protoscoleces and metacestodes. Protoscoleces
and groups of ten peritoneal cysts obtained from BALB/c
mice were incubated with the two drugs, either separately
or in combination, at the following final concentrations:
10 ìg/mL FLBZ, 1 ìg/mL FLBZ, 1 ìg/mL IVM, 10 ìg/
mL FLBZ + 1 ìg/mL IVM, and 1 ìg/mL FLBZ + 1 ìg/
mL IVM. The maximum protoscolicidal effect was found
with the combination 10 ìg/mL FLBZ + 1 ìg/mL IMV.
After 1 day of incubation, the presence of numerous blebs
in the tegument of protoscoleces was observed. Ultrastructural
studies revealed that the primary site of damage
was the tegument of the parasite. The effect of the two
drugs on hydatid cysts obtained from mice was more
rapidly detected in cysts treated with the combination of
FLBZ + IVM than when drugs were used separately.
Ultrastructural studies revealed that the germinal layer of
treated cysts lost the multicellular structure feature and
underwent considerable degenerative changes after in
vitro treatment. The outcomes obtained demonstrated the
favorable effect of the combination of FLBZ and IVM
against E. granulosus.protoscoleces and metacestodes. Protoscoleces
and groups of ten peritoneal cysts obtained from BALB/c
mice were incubated with the two drugs, either separately
or in combination, at the following final concentrations:
10 ìg/mL FLBZ, 1 ìg/mL FLBZ, 1 ìg/mL IVM, 10 ìg/
mL FLBZ + 1 ìg/mL IVM, and 1 ìg/mL FLBZ + 1 ìg/
mL IVM. The maximum protoscolicidal effect was found
with the combination 10 ìg/mL FLBZ + 1 ìg/mL IMV.
After 1 day of incubation, the presence of numerous blebs
in the tegument of protoscoleces was observed. Ultrastructural
studies revealed that the primary site of damage
was the tegument of the parasite. The effect of the two
drugs on hydatid cysts obtained from mice was more
rapidly detected in cysts treated with the combination of
FLBZ + IVM than when drugs were used separately.
Ultrastructural studies revealed that the germinal layer of
treated cysts lost the multicellular structure feature and
underwent considerable degenerative changes after in
vitro treatment. The outcomes obtained demonstrated the
favorable effect of the combination of FLBZ and IVM
against E. granulosus.E. granulosus.