INVESTIGADORES
MENSCH Julian
artículos
Título:
Gene-by-temperature interactions and candidate plasticity genes for morphological traits in Drosophila melanogaster.
Autor/es:
CARREIRA VALERIA; IMBERTI MARCOS; MENSCH JULIÁN; FANARA JUAN JOSÉ
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2013 p. 1 - 10
ISSN:
1932-6203
Resumen:
Understanding the genetic architecture of any quantitative trait
requires identifying the genes involved in its expression in different
environmental conditions. This goal can be achieved by mutagenesis
screens in genetically tractable model organisms such as Drosophila
melanogaster. Temperature during ontogenesis is an important
environmental factor affecting development and phenotypic variation in
holometabolous insects. In spite of the importance of phenotypic
plasticity and genotype by environment interaction (GEI) for fitness
related traits, its genetic basis has remained elusive. In this context,
we analyzed five different adult morphological traits (face width, head
width, thorax length, wing size and wing shape) in 42 co-isogenic
single P-element insertional lines of Drosophila melanogaster raised at
17°C and 25°C. Our analyses showed that all lines differed from the
control for at least one trait in males or females at either
temperature. However, no line showed those differences for all traits in
both sexes and temperatures simultaneously. In this sense, the most
pleiotropic candidate genes were CG34460, Lsd-2 and Spn. Our analyses
also revealed extensive genetic variation for all the characters mostly
indicated by strong GEIs. Further, our results indicate that GEIs were
predominantly explained by changes in ranking order in all cases
suggesting that a moderate number of genes are involved in the
expression of each character at both temperatures. Most lines displayed a
plastic response for at least one trait in either sex. In this regard,
P-element insertions affecting plasticity of a large number of traits
were associated to the candidate genes Btk29A, CG43340, Drak and jim.
Further studies will help to elucidate the relevance of these genes on
the morphogenesis of different body structures in natural populations of
D. melanogaster.