LONG-TERM EFFECTS OF POLYCYSTIC OVARY SYNDROME ON THE RAT UTERUS

BRACHO GS; ACOSTA MV; IÑIGUEZ I; LUQUE EH; BOSQUIAZZO VL

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022 LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

Polycystic ovary syndrome (PCOS) is the most common endocrinopathy in women of reproductive age. It is known that in PCOS, the hormonal imbalance could increase the risk of developing uterine alterations such as endometrial hyperplasia or even cancer. The aim of this study was to investigate the long-term effects of PCOS on the rat uterus. For this, we evaluated a) serum steroid hormone profiles and b) incidence and multiplicity of uterine abnormalities. To induce PCOS, Wistar female rats were injected subcutaneously with dehydroepiandrosterone (6mg/100g body weight, PCOS group) from 21 to 40 days of age. The CONTROL group received oil sesame injections. After the treatment, the animals were allowed to grow until adulthood (18-24 months approximately) and were euthanized in vaginal diestrous. Blood samples were collected to evaluate the serum levels of sex steroids and the uterine horns were dissected and processed for histological studies. All PCOS rats presented detectable levels of 17β-estradiol (E2) compared to 77.8% of CONTROL rats. In addition, the PCOS group showed a higher serum E2 levels (CONTROL: 10,7 ± 2,7 vs. PCOS: 17,6 ±1,9 pg/mL, p