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CASTROGIOVANNI Daniel Cayetano
congresos y reuniones científicas
Título:
Impact of fructose rich diet (FRD) administration in the early post-pubertal progesterone-primed female rat.
Autor/es:
DANIEL CASTROGIOVANNI, ANA ALZAMENDI ,ROLF C. GAILLARD ,EDUARDO SPINEDI, ANDRES GIOVAMBATTISTA
Lugar:
EEUU
Reunión:
Congreso; OBESITY 2009; 2009
Resumen:
Impact of Fructose Rich Diet (FRD) Administration in the Early
Post-Pubertal, Progesterone-Primed Female Rat
Daniel Castrogiovanni, Ana Alzamendi Argentina; Rolf C. Gaillard Switzerland;
Eduardo Spinedi, Andres Giovambattista ArgentinaArgentina; Rolf C. Gaillard Switzerland;
Eduardo Spinedi, Andres Giovambattista ArgentinaArgentina
The aim of this study was to evaluate the effect of FRD administration in
rats primed (at age 60 days) with progesterone (P4; im, 2 mg/200 ml corn
oil) or vehicle (controls; CT). Individually housed rats from CT and P4
groups (n = 7/8 rats per group) received commercial pellet-diet and water
ad libitum between ages 6099 days. Between days 100 and 120 of age, rats
were ad libitum fed with a FRD (10% w/v fructose in water) for drinking
and commercial-pellet diet until experimentation. Rat body weight (BW)
and food intake were daily monitored between days 60 and 99 in CT and P4
rats; and, BW, food intake and fl uid intake were daily monitored between
days 100 and 120 in CT-FRD and P4-FRD rats. Rats were euthanized
(age 120 days, 09000930 h) and trunk blood was collected; immediately
after parametrial (PM) fat pads were dissected, weighted and kept frozen.
Plasma concentrations of various metabolic markers were measured. Our
results indicate that no differences among groups accounted for BWs, either
when examined before or after FRD administration. Normalized PM fat
pad weight (g/100 g BW) and average of total daily calorie intake (even
when discriminated by source) were similar P4-FRD and CT-FRD rats.
While P4-FRD displayed enhanced plasma total cholesterol levels (P < 0.05
vs. CT-FRD rats), circulating concentrations of nonesterifi ed fatty acid,
triglyceride, glucose, insulin and adiponectin were similar in both groups.
Conversely, the plasmatic levels of leptin and plasminogen activator inhibitor-
1 (expressed in ng/ml, or normalized as in either ng/ml/100 g BW or
ng/ml/g PM fat) were signifi cantly (P < 0.05) higher and lower, respectively,
in P4-FRD than in CT-FRD rats. Interestingly, PM fat leptin and
adiponectin mRNAs were signifi cantly (P < 0.05) higher in P4-FRD than in
CT-FRD rats. Our data indicate that various adaptive metabolic mechanisms
involved in FRD intake-induced allostasis seem to be dependent on
a previously and transiently established high P4 endogenous environment.
Importantly, and because of certain paracrine effects, a distorted profi le of
PM fat adipokine gene expression could be playing a modulatory role in the
female reproductive function, at early post-pubertal age. Thus, this study
strongly encourages researchers to further develop studies on the effect of
sex steroid-diet interaction on PM adipocyte endocrine function at early
reproductive age. (Supported by PICT200701051; PIP11220080100704;
and FRPE0709)P < 0.05
vs. CT-FRD rats), circulating concentrations of nonesterifi ed fatty acid,
triglyceride, glucose, insulin and adiponectin were similar in both groups.
Conversely, the plasmatic levels of leptin and plasminogen activator inhibitor-
1 (expressed in ng/ml, or normalized as in either ng/ml/100 g BW or
ng/ml/g PM fat) were signifi cantly (P < 0.05) higher and lower, respectively,
in P4-FRD than in CT-FRD rats. Interestingly, PM fat leptin and
adiponectin mRNAs were signifi cantly (P < 0.05) higher in P4-FRD than in
CT-FRD rats. Our data indicate that various adaptive metabolic mechanisms
involved in FRD intake-induced allostasis seem to be dependent on
a previously and transiently established high P4 endogenous environment.
Importantly, and because of certain paracrine effects, a distorted profi le of
PM fat adipokine gene expression could be playing a modulatory role in the
female reproductive function, at early post-pubertal age. Thus, this study
strongly encourages researchers to further develop studies on the effect of
sex steroid-diet interaction on PM adipocyte endocrine function at early
reproductive age. (Supported by PICT200701051; PIP11220080100704;
and FRPE0709)P < 0.05) higher and lower, respectively,
in P4-FRD than in CT-FRD rats. Interestingly, PM fat leptin and
adiponectin mRNAs were signifi cantly (P < 0.05) higher in P4-FRD than in
CT-FRD rats. Our data indicate that various adaptive metabolic mechanisms
involved in FRD intake-induced allostasis seem to be dependent on
a previously and transiently established high P4 endogenous environment.
Importantly, and because of certain paracrine effects, a distorted profi le of
PM fat adipokine gene expression could be playing a modulatory role in the
female reproductive function, at early post-pubertal age. Thus, this study
strongly encourages researchers to further develop studies on the effect of
sex steroid-diet interaction on PM adipocyte endocrine function at early
reproductive age. (Supported by PICT200701051; PIP11220080100704;
and FRPE0709)P < 0.05) higher in P4-FRD than in
CT-FRD rats. Our data indicate that various adaptive metabolic mechanisms
involved in FRD intake-induced allostasis seem to be dependent on
a previously and transiently established high P4 endogenous environment.
Importantly, and because of certain paracrine effects, a distorted profi le of
PM fat adipokine gene expression could be playing a modulatory role in the
female reproductive function, at early post-pubertal age. Thus, this study
strongly encourages researchers to further develop studies on the effect of
sex steroid-diet interaction on PM adipocyte endocrine function at early
reproductive age. (Supported by PICT200701051; PIP11220080100704;
and FRPE0709)n = 7/8 rats per group) received commercial pellet-diet and water
ad libitum between ages 6099 days. Between days 100 and 120 of age, rats
were ad libitum fed with a FRD (10% w/v fructose in water) for drinking
and commercial-pellet diet until experimentation. Rat body weight (BW)
and food intake were daily monitored between days 60 and 99 in CT and P4
rats; and, BW, food intake and fl uid intake were daily monitored between
days 100 and 120 in CT-FRD and P4-FRD rats. Rats were euthanized
(age 120 days, 09000930 h) and trunk blood was collected; immediately
after parametrial (PM) fat pads were dissected, weighted and kept frozen.
Plasma concentrations of various metabolic markers were measured. Our
results indicate that no differences among groups accounted for BWs, either
when examined before or after FRD administration. Normalized PM fat
pad weight (g/100 g BW) and average of total daily calorie intake (even
when discriminated by source) were similar P4-FRD and CT-FRD rats.
While P4-FRD displayed enhanced plasma total cholesterol levels (P < 0.05
vs. CT-FRD rats), circulating concentrations of nonesterifi ed fatty acid,
triglyceride, glucose, insulin and adiponectin were similar in both groups.
Conversely, the plasmatic levels of leptin and plasminogen activator inhibitor-
1 (expressed in ng/ml, or normalized as in either ng/ml/100 g BW or
ng/ml/g PM fat) were signifi cantly (P < 0.05) higher and lower, respectively,
in P4-FRD than in CT-FRD rats. Interestingly, PM fat leptin and
adiponectin mRNAs were signifi cantly (P < 0.05) higher in P4-FRD than in
CT-FRD rats. Our data indicate that various adaptive metabolic mechanisms
involved in FRD intake-induced allostasis seem to be dependent on
a previously and transiently established high P4 endogenous environment.
Importantly, and because of certain paracrine effects, a distorted profi le of
PM fat adipokine gene expression could be playing a modulatory role in the
female reproductive function, at early post-pubertal age. Thus, this study
strongly encourages researchers to further develop studies on the effect of
sex steroid-diet interaction on PM adipocyte endocrine function at early
reproductive age. (Supported by PICT200701051; PIP11220080100704;
and FRPE0709)P < 0.05
vs. CT-FRD rats), circulating concentrations of nonesterifi ed fatty acid,
triglyceride, glucose, insulin and adiponectin were similar in both groups.
Conversely, the plasmatic levels of leptin and plasminogen activator inhibitor-
1 (expressed in ng/ml, or normalized as in either ng/ml/100 g BW or
ng/ml/g PM fat) were signifi cantly (P < 0.05) higher and lower, respectively,
in P4-FRD than in CT-FRD rats. Interestingly, PM fat leptin and
adiponectin mRNAs were signifi cantly (P < 0.05) higher in P4-FRD than in
CT-FRD rats. Our data indicate that various adaptive metabolic mechanisms
involved in FRD intake-induced allostasis seem to be dependent on
a previously and transiently established high P4 endogenous environment.
Importantly, and because of certain paracrine effects, a distorted profi le of
PM fat adipokine gene expression could be playing a modulatory role in the
female reproductive function, at early post-pubertal age. Thus, this study
strongly encourages researchers to further develop studies on the effect of
sex steroid-diet interaction on PM adipocyte endocrine function at early
reproductive age. (Supported by PICT200701051; PIP11220080100704;
and FRPE0709)P < 0.05) higher and lower, respectively,
in P4-FRD than in CT-FRD rats. Interestingly, PM fat leptin and
adiponectin mRNAs were signifi cantly (P < 0.05) higher in P4-FRD than in
CT-FRD rats. Our data indicate that various adaptive metabolic mechanisms
involved in FRD intake-induced allostasis seem to be dependent on
a previously and transiently established high P4 endogenous environment.
Importantly, and because of certain paracrine effects, a distorted profi le of
PM fat adipokine gene expression could be playing a modulatory role in the
female reproductive function, at early post-pubertal age. Thus, this study
strongly encourages researchers to further develop studies on the effect of
sex steroid-diet interaction on PM adipocyte endocrine function at early
reproductive age. (Supported by PICT200701051; PIP11220080100704;
and FRPE0709)P < 0.05) higher in P4-FRD than in
CT-FRD rats. Our data indicate that various adaptive metabolic mechanisms
involved in FRD intake-induced allostasis seem to be dependent on
a previously and transiently established high P4 endogenous environment.
Importantly, and because of certain paracrine effects, a distorted profi le of
PM fat adipokine gene expression could be playing a modulatory role in the
female reproductive function, at early post-pubertal age. Thus, this study
strongly encourages researchers to further develop studies on the effect of
sex steroid-diet interaction on PM adipocyte endocrine function at early
reproductive age. (Supported by PICT200701051; PIP11220080100704;
and FRPE0709)