Estudios Biológicos in Vitro de materiales utilizados en osteosíntesis

CASTROGIOVANNI, D.; PARISI J.; REIGOSA, M.

Revista de la Facultad de Ciencias Medicas de Quito

Facultad de Medicina

Año: 2014 vol. 39 p. 15 - 21

0375-1066

AbstractContext: some materials are used to factory medical prosthesis, in repairing medicine. Titanium is the most common used material bi their chemical characteristics. Aim: to evaluate biocompatibility in different samples of medical prosthesis, manufactured by FICO S.A. enterprise.Methods: through cytotoxicity and genotoxicity techniques, it analyzed the potential capability to integrate into the tissues, working with cell cultures and cell lines UMR-106 y L929. Also it used techniques such as, neutro red, colorimetric of MTT, direct contact, diffusion on agar and collagen production, it evaluated the cytotoxicity levels. Also with acridine orange technique it sets cell viability. Comet essay was used to establish genotocicity. Results: the study shows high biocompatibility, and it does not found significant difference in negative controls. Conclusion: It recommends these techniques to evaluate biomedical prosthesis.