PERSONAL DE APOYO
BERNARDELLI Cecilia Elena
artículos
Título:
PERIPLASMIC PQQ-DEPENDENT GLUCOSE OXIDATION IN FREE-LIVING AND SYMBIOTIC RHIZOBIA
Autor/es:
BERNARDELLI CECILIA; LUNA MARÍA F; GALAR, ML; BOIARDI, JL
Revista:
CURRENT MICROBIOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2001 vol. 42 p. 310 - 315
ISSN:
0343-8651
Resumen:
Abstract. The expression of the pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) of Rhizobium tropici CIAT899 and inorhizobium meliloti RCR2011 was investigated under different nutrient-limiting conditions in continuous cultures, under different conditions of phosphate availability, and in S. meliloti bacteroids. The presence of free PQQ in alfalfa root exudates has also been assayed. It was shown that apo-GDH or holoenzyme was actively synthesized by these rhizobia, with the concomitant production of gluconate from glucose, under certain environmental conditions. GDH activity was also detected in bacteroids from alfalfa root nodules inoculated with either S. meliloti RCR2011 or 102F34. It was also shown that free PQQ was present in root exudates of alfalfa, but its production is ascribed to the activity of Erwinia sp., a normal contaminant of these seeds.
