CHANGES IN THE CONTENT OF NEUROPEPTIDE GLUTAMIC ACID ISOLEUCINE (NEI) IN DIFFERENT BRAIN AREAS SECONDARY TO THE INDUCTION OF HYPO- AND HYPERTHYROIDISM IN ADULT MALE RATS

AYALA CAROLINA; NAVARRA MORERO ML; SOAJE MARTA; VALDEZ SUSANA R; CARREÑO NORMA B; JAHN, GRACIELA A; CELIS

Ruen, Francia

Congreso; THE 7TH INTERNATIONAL CONGRESS OF NEUROENDOCRINOLOGY International Neuroendocrine Federation (INF) – Société de Neuroendocrinologie (SNE); 2010

Neuroendocrine Federation (INF) – Société de Neuroendocrinologie (SNE)

@font-face { font-family: "Cambria"; }@font-face { font-family: "Calisto MT"; }p.MsoNormal, li.MsoNormal, div.MsoNormal { margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: "Times New Roman"; }div.Section1 { page: Section1; } Introduction: NEI is a linear 13-aminoacids neuropeptide derived from pp-MCH. NEI is able to inhibit TRH release in mediobasal hypothalamus and to stimulate LH release in hypothalamus and pituitary. Aim: To study whether hypo- and/or hyperthyroidism may change the content of the peptide in different brain areas. Materials and Methods: Adult male Wistar rats divided into control (CTR) and treated (T):  hypothyroid animals (PTU) received PTU 0.1 g/l in drinking water during 8th days, hyperthyroid induced group (T4) received L-T4 250 μg/kg sc daily for 4 days. Animals were sacrificed in the morning (M) and afternoon (A). Trunk blood was collected; pineal gland, brain and posterior pituitary (PP) were obtained. Coronal brain slices of 1 mm were serially sectioned and the areas of interest were collected by microdissection. NEI content was determined by radioimmunoassay (RIA). Statistics: one-way ANOVA followed by Tukey’s test (Tukey’s HDS), significance p < 0.05. Results: anteroventral periventricular nucleus: higher values in PTU at M; preoptic area: increased content in T4 at M; lateral hypotalamic area+perifornix: lower amount in T4 at M; zona incerta+IHy: higher values in T4 at M;  median eminence+arcuate nucleus: higher values of the peptide at M in T4;  PP: higher content of NEI in T4 at M. Discussion: The higher amount of NEI in the T4 group may allow as to propose that higher levels of thyroid