Effect of hyperthyroidism on circulating prolactin and hypothalamic expresión of tyrosine hydroxylase, prolactin signaling cascade members and estrogen and progesterone receptors during late pregnancy and lactation in the rat

PENNACCHIO GISELA E; NEIRA FLAVIA J; SOAJE MARTA; JAHN GRACIELA A; VALDEZ SUSANA R

MOLECULAR AND CELLULAR ENDOCRINOLOGY.

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2017 vol. 442 p. 40 - 50

0303-7207

<!-- /* Font Definitions */@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} /* Style Definitions */p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Times New Roman";mso-ascii-font-family:Cambria;mso-fareast-font-family:Cambria;mso-hansi-font-family:Cambria;mso-bidi-font-family:"Times New Roman";mso-fareast-language:EN-US;}@page Section1{size:595.0pt 842.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:35.4pt;mso-footer-margin:35.4pt;mso-paper-source:0;}div.Section1{page:Section1;}-->Effect of hyperthyroidism on circulatingprolactin and hypothalamic expresión of tyrosine hydroxylase, prolactinsignaling cascade members and estrogen and progesterone receptors during late pregnancyand lactation in the rat. Gisela E. Pennacchio, Flavia J.Neira, Marta Soaje, Graciela A. Jahn,Susana R. Valdez. Hyperthyroidism(HyperT) compromises pregnancy and lactation, hindering suckling-induced PRLrelease. We studied the effect of HyperT on hypothalamic mRNA (RT-qPCR) andprotein (Western blot) expression of tyrosine hydroxylase (TH), PRL receptor(PRLR) and signaling pathway members, estrogena (ERa) and progesterone (PR) receptors on latepregnancy (days G19, 20 and 21) and early lactation (L2) in rats. HyperTadvanced pre-partum PRL release, reduced circulating PRL on L2 and increased THmRNA (G21 and L2), p-TH, PRLR mRNA, STAT5 protein (G19 and L2), PRLR protein(G21) and CIS protein (G19). PRs mRNAs and protein decreased on G19 butafterwards PRA mRNA (G20), PRB mRNA (G21) and PARA mRNA and protein (L2)increased. ERa protein increased on G19 and decreased on G20. Thus, the alteredhypothalamic PRLR, STAT5, PR and ERa expression in hyperthyroid rats may induceelevated TH expression and activation, that consequently, elevate dopaminergictone during lactation, blunting suckling-induced PRL release and litter growth.