The immediate early protein c-Fos has been extensively described as a member of the AP-1 family of transcription factors. As such, it has been implicated in cellular events such as differentiation and proliferation. All data available failed to completely

FERRERO GO AND CAPUTTO BL

Potrero de Funes

Congreso; Reunión de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2011

SAIB

The immediate early protein c-Fos has been extensively described as a member of the AP-1 family of transcription factors. As such, it has been implicated in cellular events such as differentiation and proliferation. All data available failed to completely explain each particular process. In our lab an aditional activity for c-Fos was described that could eventually complete the story. c-Fos has the ability to activate the phospholipid synthesis machinery within the cytoplasm in an AP-1 independent manner. In the present report we show that PI4P and PI-4,5-P2 nuclear synthesis is also affected recombinant c-Fos the addition of to the nuclear matrix model results in wide transcriptional changes (as determinated by RNA array). This changes were confirmed by qPCR in nuclear matrix and whole cell. A two fold increase in nuclear matrix size was observed upon treatment with c-Fos along with the apparition of PIP2 patches in specific locations, indicating that major structural changes are taking place in response to c-Fos addition. Nevertheless, if wortmannin is present in the incubated, all mentioned changes are revoked. This report establish that c-Fos transcriptional regulatory activities may not always be linked to AP-1 dimers; rather c-Fos induce can AP-1 independent changes, resulting in wide global transcriptional changes mediated by phosphoinositides.